2,3,5-Triphenyl-2H-tetrazolium chloride

In contrast with previous LTV spectrophotometric method, Khan et al. [17] reported first -derivative spectrophotometric method to determine glimepiride in pharmaceutical dosage form. In this method, formation of drug complex was carried out by reacting glimepiride in MeOH with 2,3,5-triphenyl-2H-tetrazolium chloride in NaOH 0.1 M as an alkaline media. This mixture was then heated at 60 2 °C for 60 min. Drug complex formed was showing maximum absorption at... 

Triphenyl-2H-tetrazolium chloride. Available from Aldrich and Eastman. Light-sensitive. Freshly prepared aqueous solutions should be used in tests. Any unused solution should be acidified and discarded. 

TPTZ 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate TTC... 

Triphenylstannium acetate. See Fentin acetate Triphenylstibine. See Triphenylantimony 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate. See Tetrazolium chloride Triphenyl thiophosphate 0,0,0-Triphenyl thiophosphate. See Triphenyl phosphorothionate... 

HPLC method was previously rqmrted (5). Two mL of AGE was applied into an ion exchange column cmitaining 4 mL of Dowex 50Wx8 (NHt form) resin. After washing with 40 mL of water, the sample solution was eluted with 0.2 M ammonium hydroxide (20 mL). The effluent was appropriately diluted with 0.2 M ammonium hydroxide, if necessary. The IffLC stem was a LCIOVP (Shimadzu, Kyoto, Japan) witii reaction coil heater CRB-6A (Shimadzu, Kyoto, Japan). HPLC conditimis wwe as follows, column Shodex Asahipak NH2P-50 4E (Showa Denko K.K., Tokyo, Japan) mobile phase acetonitrile-10 mM potassium-phosphate buffer (pH 6.7) (31 19, v/v), 1.0 mL/min reaction solution 2,3,5-triphenyl-2H-tetrazolium chloride 2.0 g/L in 100 mM sodium hydroxide-ethanol (1 1, v/v), 0.2 mL/min reaction coil 95 C, 0.5 mm i.d. x 5 m detection absorbance at 480 nm injection volume 10 pL column temperature ambient. 

The zones or spots of separated compormds are generally visualized by detecting dehydrogenase activity with tetrazolium salt-based reagents. The metabolically active bacteria convert the tetrazolium salt into red formazan dye (2,3,5-triphenyl-2H-tetra-zolium chloride, tetrazolium red). A number of tetrezolium salts were evaluated as potential substrates for the enzymatic reaction, but p-iodonitrote-trazolium violet, tetranitro blue tetrazolium, and MTT were found to be suitable substrates. The equations below show the principle of the reaction with tetrazolium salt, 3-[4,5-dimethylthiazol-2-yl]-2,5 dip-henyltetrazolium bromide (MTT, tetrazolium dye). 

H-Tetrazole-5-thiol, 1-phenyl. See 1-Phenyl-5-mercaptotetrazole Tetrazolium chloride CAS 298-96-4 EINECS/ELINCS 206-071-6 Synonyms PTB Red tetrazolium Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride... 

Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride. See Tetrazolium chloride... 

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