2,3,5-Triphenyl-2H-tetrazolium

In contrast with previous LTV spectrophotometric method, Khan et al. [17] reported first -derivative spectrophotometric method to determine glimepiride in pharmaceutical dosage form. In this method, formation of drug complex was carried out by reacting glimepiride in MeOH with 2,3,5-triphenyl-2H-tetrazolium chloride in NaOH 0.1 M as an alkaline media. This mixture was then heated at 60 2 °C for 60 min. Drug complex formed was showing maximum absorption at... 

Triphenyl-2H-tetrazolium chloride. Available from Aldrich and Eastman. Light-sensitive. Freshly prepared aqueous solutions should be used in tests. Any unused solution should be acidified and discarded. 

TPTZ 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate TTC... 

Triphenylstannium acetate. See Fentin acetate Triphenylstibine. See Triphenylantimony 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate. See Tetrazolium chloride Triphenyl thiophosphate 0,0,0-Triphenyl thiophosphate. See Triphenyl phosphorothionate... 

Various tetrazolium compounds, introduced into enzyme research by Kuhn and Jerchel (1941) and Lakon (1942), are reduced to scarlet-red (2,3,5-triphenyl-2H-tetrazolium), purplish (neoetrazo-lium) or blue (ditetrazolium) formazans, insoluble in water and soluble in fats. Formazan generation is practically inhibited by superoxide dismutase as well as by catalase (Bromme et al. 1999). When superoxide radical generation via the xanthine-xanthine oxidase system was measured by chemiluminescence an addition of nitroblue tetrazolium immediately abolished lucigenin-enhanced chemiluminescence. Nitroblue tetrazolium did not inhibit xanthine oxidase. 

HPLC method was previously rqmrted (5). Two mL of AGE was applied into an ion exchange column cmitaining 4 mL of Dowex 50Wx8 (NHt form) resin. After washing with 40 mL of water, the sample solution was eluted with 0.2 M ammonium hydroxide (20 mL). The effluent was appropriately diluted with 0.2 M ammonium hydroxide, if necessary. The IffLC stem was a LCIOVP (Shimadzu, Kyoto, Japan) witii reaction coil heater CRB-6A (Shimadzu, Kyoto, Japan). HPLC conditimis wwe as follows, column Shodex Asahipak NH2P-50 4E (Showa Denko K.K., Tokyo, Japan) mobile phase acetonitrile-10 mM potassium-phosphate buffer (pH 6.7) (31 19, v/v), 1.0 mL/min reaction solution 2,3,5-triphenyl-2H-tetrazolium chloride 2.0 g/L in 100 mM sodium hydroxide-ethanol (1 1, v/v), 0.2 mL/min reaction coil 95 C, 0.5 mm i.d. x 5 m detection absorbance at 480 nm injection volume 10 pL column temperature ambient. 

The zones or spots of separated compormds are generally visualized by detecting dehydrogenase activity with tetrazolium salt-based reagents. The metabolically active bacteria convert the tetrazolium salt into red formazan dye (2,3,5-triphenyl-2H-tetra-zolium chloride, tetrazolium red). A number of tetrezolium salts were evaluated as potential substrates for the enzymatic reaction, but p-iodonitrote-trazolium violet, tetranitro blue tetrazolium, and MTT were found to be suitable substrates. The equations below show the principle of the reaction with tetrazolium salt, 3-[4,5-dimethylthiazol-2-yl]-2,5 dip-henyltetrazolium bromide (MTT, tetrazolium dye). 

H-Tetrazole-5-thiol, 1-phenyl. See 1-Phenyl-5-mercaptotetrazole Tetrazolium chloride CAS 298-96-4 EINECS/ELINCS 206-071-6 Synonyms PTB Red tetrazolium Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride... 

Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride. See Tetrazolium chloride... 

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