Tetrahymena pyriformis

Toyopearl HW-50S resin has been used to help isolate the ubiquitin-histone conjugate mH2A from the unicellular ciliated protozoan Tetrahymena pyriformis. Figure 4.49 shows the separation of mH2A from the histone, H2A. The sole difference between these two components is a small polypeptide, ubiquitin (approximately 8500 Da). The mH2A fraction was then further purified by HPLC on a Tosoh ODS-silica column (52). One of the many benefits... 

Tipton CL, NM Al-Shathir (1974) The metabolism of cyclopropane fatty acids by Tetrahymena pyriformis. J Biol Chem 249 886-889. 

TEST allows for estimates of the value for several toxicity endpoints [29] 96 h Fathead minnow LC50, 48 h Daphnia magna LC50, 48 h Tetrahymena pyriformis IGC50, Oral rat LD50, bioaccumulation factor, developmental toxicity, and Ames mutagenicity. TEST also estimates several physical properties... 

Values of molecular weight (uncorrected for dissymmetry) have been obtained by Harrap and Manners238 from light-scattering investigations, as follows rabbit liver, 6.8 X 106 rabbit muscle, 2.8 X 109 cat liver, 10.0 X 109 fetal sheep liver, 14.8 X 106 Tetrahymena pyriformis, 9.8 X 106 and Ascaris lumbricoides, 8.8 X 106. 

Boisson, F., M. Gnassia-Barelli, and M. Romeo. 1995. Toxicity and accumulation of selenite and selenate in the unicellular marine algae Cricosphaera elongata. Arch. Environ. Contamin. Toxicol. 28 497-493. Bovee, E.C. and T.L. O Brien. 1982. Some effects of selenium, vanadium and zirconium on the swimming rate of Tetrahymena pyriformis a bioassay study. Univ. Kans. Sci. Bull. 52 (4) 39 44. 

Tetrahymena pyriformis utilizes nicotinic acid or nicotinamide when they are added in the same concentration, the increment of growth is less than that predicted from the algebraic sums of the individual increments (Table 5). 

Fig. 4. Growth of Tetrahymena pyriformis with members of the vitamin Be group.

A new synthesis (Scheme 4) of 2-aminoethylphosphonic acid (24) has been described in which bis(dimethylamino) phosphorochloridate is condensed with an a-anion derived from an aliphatic nitrile.71 An advantage of this synthesis is that the intermediate (25) can be alkylated, which constitutes the simplest synthesis so far published for substituted aminoethylphosphonic acids. Both C-3 and the phosphorus atom of PEP are incorporated into (24) in Tetrahymena pyriformis,72 which confirms... 

Toropov AA, Benfenati E (2004) QSAR modelling of aldehyde toxicity against a protozoan, Tetrahymena pyriformis by optimization of correlation weights of nearest neighboring codes. J. Mol. Struct. (Theochem) 679 225-228. 

H. Tanabe, N. Kamo, and Y. Kobatake, Fluorometric estimation of surface potential change associated with chemotactic stimulation in Tetrahymena pyriformis, Biochim. Biophys. Acta 805, 345-353 (1984). 

Muccini, M., Layton, A.C., Sayler, G.S., and Schultz, T.W. Aquatic toxicities of halogenated benzoic acids to Tetrahymena pyriformis, Bull. Environ. Contam. Toxicol, 62(5) 616-622, 1999. 

As protozoa and nematodes live in pore water in the soil, most of the methods are adapted from toxicity tests designed for aquatic samples. Among the protozoa the tests with cihates Tetrahymena pyriformis, Tetrahymena thermophiia, Colpoda cucullus, Colpoda inflata, Colpoda steinii, Paramecium caudatum, and Paramecium aurelia have been developed [ 102,112-117]. It is the opinion of some authors that the sensitivity of infusorians is higher than that of microorganisms [115,116]. 

Bogaerts, P. Bohatier, J. Bonnemoy, E. Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of the toxicity and quantitative structure-activity relationships of xenobiotics comparison with the microtox test. Ecotox. Environ. Safe. 2001, 49, 293-301. 

Burden, F. R., and Winkler, D. A. (2000) A quantitative structure-activity relationships model for the acute toxicity of substituted benzenes to Tetrahymena pyriformis using Bayesian-regularized neural networks. Chem. Res. Toxicol. 13,436-440. 

Zhu, H., Tropsha, A., Fourches, D., Varnek, A., Papa, E., Gramatica, P., Oberg, T., Dao, P., Cherkasov, A. and Tetko, I.V. (2008) Combinatorial QSAR modeling of chemical toxicants tested against Tetrahymena pyriformis. Journal of Chemical Information and Computer Sciences, 48, 766-784. 

Perkins, D. L., and L. S. Ciereszko. Effect of macrocyclic diterpene from tobacco on Tetrahymena pyriformis. 

The identity of a glucosylated derivative of L-biopterin from Synechococcus sp. PGG 7942 has been confirmed as the 2 -0-a-glucosyl derivative by 2-D NMR spectroscopy <2001PTR121>. Tetrahydro-D-monapterin 223 has been identified as the native pteridine in Tetrahymena pyriformis <2001HGA918> the configuration and stmcture were confirmed by comparison with synthetic samples of known configuration. 

As shown in Scheme 1, two other pathways lead to the formation of dolichol phosphate. These are (a) phosphorylation of the free poly-prenol, and (b) dephosphorylation of dolichol diphosphate. A dolichol phosphate-cleaving phosphatase has been described as occurring in Tetrahymena pyriformis,15 human lymphocytes,18 calf brain,17 and rat-nerve tissue.18 The protozoal enzyme was a soluble acid phosphatase, although it was clearly different from the bulk phosphatase activity, whereas the mammalian enzymes had optima at neutral pH and were membrane-bound. 

Tunicamycin specifically inhibits cell division and pairing between the mating types of Tetrahymena pyriformis. The glycoproteins involved in this mating process have not yet been characterized, but may coincide with new, concanavalin A receptor-sites that appear during conjugation.495... 

A complete synthetic media for the growth of Tetrahymena pyriformis E., a protozoan whose nutritional requirements closely simulate those of mammals, was irradiated at Michigan (B16). When the medium failed to support the growth of Tetrahymena, the essential vitamins and amino acids were individually irradiated in solution. All vitamins were altered structurally by less than sterilization doses. The amino acids proved stable under the same conditions. 

Anderson, E.P. UMP-CMP kinase from Tetrahymena pyriformis. Methods EnzymoL, 51, 331-337 (1978)... 

Gravey, T.Q. Millar, F.K. Anderson, E.P. ATP UMP-CMP phosphotransferase from Tetrahymena pyriformis. IL Kinetic studies and reaction mechanism with UMP. Biochim. Biophys. Acta, 302, 38-49 (1973)... 

Cronin, M.T.D. and Schultz, T.W. 2001. Development of quantitative structure-activity relationships for the toxicity of aromatic compounds to Tetrahymena pyriformis Comparative assessment of the methodologies. Chem. Res. Toxicol. 14 1284-1295. 

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