Tetrahymena pyriformis cultures

Yamaguchi, N., O. Wad a, T. Ono, and K. Yazaki Detection of heavy metal toxicity by Tetrahymena pyriformis culture method. Ind. Health 11, 27 (1973). 

The resolution of synthetic presqualene and prephytoene alcohols via their etienic acid derivatives has been reported. This work confirmed that the active (-f-)-enantiomers in both series have the same absolute configuration [(li , 2/ , 3/ )]. It has been established, by use of Hn.m.r., that the proton (deuteron) introduced at C-3 during the cyclization of squalene to tetrahymanol by Tetrahymena pyriformis has the 3/8 configuration. Both antipodes of the trimethyldecalol (13) have been shown to be effective inhibitors of cholesterol biosynthesis in rat liver enzyme preparations and cultured mammalian cells. The accumulation of squalene 2,3-oxide and squalene 2,3 22,23-dioxide in the treated systems indicates that inhibition occurs at the cyclization stage. The inhibitor is metabolized to the diol (14). The results of other sterol inhibition... 

Geldanamycin inhibited the growth and multiplication of protozoa, being primarily active against Tetrahymena pyriformis and Crithidia fasciculata. It was also active against L-1210 and KB cells growing in culture, and against the parasite Syphacia... 

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