Tetrahymena pyriformis material

Extraction of Insulin-Related Material and Other Peptide Hormones from Tetrahymena pyriformis... 

Figure 3. Sep-Pak (C g) purification of insulin-related material. I-insulin and purified porcine insulin were added to separate batches of Tetrahymena pyriformis homogenates and applied to Sep-Pak cartridges. Following elution from the Sep-Pak each gradient fraction was tested for recovery of the exogenously added insulin. The results for each fraction are expressed as the percent of the total material recovered. Key , endogenous insulin Tetrahymena , 125i-insulin added to extract 0, porcine insulin added to extract.

Figure 6. Somatostatin-like material in Tetrahymena pyriformis. Key (right panel) , synthetic somatostatin O, Tetrahymena extract (HPLC purified) Band , anti-somatostatin antibody added. (Reproduced from Ref. 8.)...

Figure 7. ACTH-like material in Tetrahymena pyriformis. (bottom panel) , the extract x, synthetic human ACTH standard O, anti-ACTH antiserum , Sepharose-protein A immunodepletion. (Reproduced from Ref. 5).

In Tetrahymena pyriformis the formation of the A -double bond involves the loss of the 6a-hydrogen atom. With [6a- H]cholest-7-en-3/3-ol a substantial isotope effect was noted with the recovered starting material. 

Subcellular distribution of endogenous T. pyriformis insulinlike material differed from the subcellular distribution of 12 I-insulin and purified porcine insulin which had been added to Tetrahymena cells prior to subcellular fractionation 07). 

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