Tetracycline inducible

Note Doses may be increased for more severe disease and may require modification in patients with organ dysfunction. Tetracyclines are rarely used in pediatric patients, particularly in those younger than 8 years of age because of tetracycline-induced permanent tooth discoloration. 

A detailed protocol for the generation of genetically modified human xenograft mouse models in which the knockdown of a putative oncology target is temporally regulated in vivo using a tetracycline inducible system is found in this book (17). 

This is the accumulation of triglycerides in hepatocytes, and there are a number of mechanisms underlying this response as is discussed below (see the sect. "Mechanisms of Toxicity"). The liver has an important role in lipid metabolism, and triglyceride synthesis occurs particularly in zone 3. Consequently, fatty liver is a common response to toxicity, often the result of interference with protein synthesis, and may be the only response as after exposure to hydrazine, ethionine, and tetracycline, or it may occur in combination with necrosis as with carbon tetrachloride. It is normally a reversible response, which does not usually lead to cell death, although it can be very serious as is the case with tetracycline-induced fatty liver in humans. Repeated exposure to compounds, which cause fatty liver, such as alcohol, may lead to cirrhosis. 

DaRocha, W.D., Otsu, K., Teixeira, S.M. and Donelson, J.E. (2004) Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi. Molecular and Biochemical Parasitology 1 33, 1 75-186. 

Blau HM, Rossi FM (1999), Tet B or not tet B advances in tetracycline-inducible gene expression, Proc. Natl Acad. Sci. USA 96 797-799. 

Tetracyclines induce photosensitisation and other rashes. Liver and pancreatic damage can occur, especially in pregnancy and with renal disease, when the drugs have been given i.v. Rarely tetracyclines cause benign intracranial hypertension, dizziness and other neurological reactions. 

A personally experienced case of likely tetracycline-induced liver injury after low-dose tetracycline has been reported (106). The patient took oral doxycycline 200 mg/ day for 8 days and had markedly altered liver function. The liver enzyme activities normalized only 109 days after withdrawal. The authors also reviewed all reports of liver damage to Swedish Adverse Drug Reactions Advisory... 

Peters RL, Edmondson HA, Mikkelsen WP, Tatter D. Tetracycline-induced fatty liver in nonpregnant patients. A report of six cases. Am J Surg 1967 113(5) 622-32. 

Hunt CM, Washington K. Tetracycline-induced bile duct paucity and prolonged cholestasis. Gastroenterology 1994 107(6) 1844-7. 

Elmore MF, Rogge JD. Tetracycline-induced pancreatitis. Gastroenterology 1981 81(6) 1134-6. 

Bihorac A, Ozener C, Akoglu E, Kullu S. Tetracycline-induced acute interstitial nephritis as a cause of acute renal failure. Nephron 1999 81(l) 72-5. 

Amacher DE, Martin B-A. Tetracycline-induced steatosis in primary canine hepa-tocyte cultures. Fund Appl Toxicol 1997 40 256-63. 

P. B. (1999) Use of the human EF-lalpha promoter for expression can significantly increase success in establishing stable cell lines with consistent expression a study using the tetracycline-inducible system in human cancer cells. Nucleic Acids Res. 27(24) 4775-4782. 

Use of dimerizers to control transcription of engineered target genes represents an alternative to technologies such as the tetracycline-inducible ( Tet ) system... 

In the original version of the tetracycline-inducible system of conditional transgenesis, tetracycline prevents transgene expression. Suggest how this tet-off system is regulated, compared with the tet-on system in Example 9.50. 

Prostaglandin E2 (PGE2) Immunoassay. Parental PC-3 cells and the PC-3 tetracycline-inducible COX-2 antisense clones with or without doxycycline (2 p.g/mL) pretreatment were propagated to 1 x 10 cells in T-75 flasks in 10% FBS-supple-... 

MiUer EJ, Kajikawa O, Pueblitz S, Light RW, Koenig KK, Idell S. Chemokine involvement in tetracycline-induced pleuritis. Eur Respir J 1999 14 1387-1393. Nasreen N, Hartman DL, Mohammed KA, Antony VB. Talc-induced expression of C-C and C-X-C chemokines and intercellular adhesion molecule-1 mesotbelial cells. Am J Respir Crit Care Med 1998 158 971-978. 

Rodriguez-Garcia, A., Combes, P, Perez-Redondo, R., Smith, M.C.A. et al (2005) Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces. Nucleic Acids Res., 33 (9), e87. 

Delaney TJ (1970) Tetracycline-induced fixed drug emptions. Br J Dermatol 83 357-358... 

Wenz B, Klein RL, Lalezari P (1974) Tetracycline-induced immune hemolytic anemia. Transfusion 14 265-269... 

Bhattachaqee SB, Pal B (1982) Tetracycline induced mutation in cultured Chinese hamster cells. Mutat Res 101 329-338... 

Morrison VL, Kikkawa DO, Herndier BG. Tetracycline induced green conjunctival pigment deposits. Br J Ophthalmol 2005 89(10) 1372-3. 

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