Tetracycline-inducible VP-CAR transgenic mice

Using this mouse model, we were able to show a novel CAR-mediated and CYP3A-independent pathway of bile acid detoxification [32], When the TRE-VP-CAR/Lap-tTA bitransgenic mice or control littermates were dosed with vehicle solvent or LCA for 4 days before liver histological evaluation, the VP-CAR 

The VP-CAR mice were also used to demonstrate the effect of CAR activation on bilirubin clearance. No differences in serum bilirubin levels were seen in untreated VP-CAR or wild-type mice. However, upon bilirubin injection, the levels of total bilirubin in the transgenic mice were less than half of those in wild-type mice [25], The protection was VP-CAR dependent, as no protection was seen in mice treated with Dox for 7 days prior to bilirubin injection [25]. Consistent with their resistance to 

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