Conditional transgenesis

In the original version of the tetracycline-inducible system of conditional transgenesis, tetracycline prevents transgene expression. Suggest how this tet-off system is regulated, compared with the tet-on system in Example 9.50. 

In rice endosperm that accumulated phytoene by virtue of daffodil Psy transgenesis, Burkhart et al. reported the failure of daffodil Pds over-expression in rice endosperm to condition accumulation of -carotene, even though an increase in PDS antigen was detected. ... 

Heinhammer A, Wurst W, Kuhn R (2011) Constitutive and conditional RNAi transgenesis in mice. Methods 53 430-436... 

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