Targeting Antigens to Specific Tissues

Tissue-specific targeting of antigens can greatly facilitate the harvesting of proteins. Streaffield et al. [28] expressed antigens specifically in maize kernels, Sandhu et al. 

Some agronomically important plant species (e. g. soybean and most cereal grains) are recalcitrant to Agrobacterium transformation, and a biolistic method (microprojectile bombardment) is frequently used for these plants [47]. DNA coated on micron-sized gold particles is propelled into plant cells using compressed helium gas 

Seed-specific production of LTB by Streatfield et al. [28] resulted in expression levels of up to 1.8% total soluble protein (TSP), and two separate maize breeding programs have increased antigen production by fivefold [28] and tenfold [27]. The investigations of Chikwamba et al. [27] regarding the expression of LTB in maize are among the first to include the use of particle bombardment transformation for the production of plant-derived vaccines. 

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