Agrobacterium transformation

Some agronomically important plant species (e. g. soybean and most cereal grains) are recalcitrant to Agrobacterium transformation, and a biolistic method (microprojectile bombardment) is frequently used for these plants [47]. DNA coated on micron-sized gold particles is propelled into plant cells using compressed helium gas... 

Host Range and Susceptibility of Plants TO Agrobacterium Transformation... 

For Agrobacterium transformation, the gene of interest must be carried in a binary vector with appropriate bacterial T-DNA elements. For other transformation methods the requirements maybe less stringent. However, even in these cases, the DNA of interest is typically amplified in bacteria. The choice of a promoter is of particular importance, as this element will dictate both the expression level and the time course of protein expression. The inclusion of additional elements, such as enhancers, 5 and 3 untranslated regions, and fusion elements (e.g. fluorescent or 6-histidine tags), should also be considered for improved expression, detection, and downstream processing. 

Palazon J, Altabella T, Cusido R, Ribo M, Pinol MT (1995) Growth and tropane alkaloid production in Agrobacterium transformed roots and derived callus of Datura. Biol Plant 37(2) 161-168... 

In order to study the processes of plant fatty acid desaturation and glycerolipid biosynthesis and to develop a crop seed oil with reduced level of saturated fatty acids, a rat liver stearyl-CoA A-9 desaturase (D9DS) gene was introduced into Nicotiana tahacum under the control of the 35S promoter via Agrobacterium transformation (3) and soybean somatic embryos with the seed-specific phaseolin promoter by particle bombardment. In this article, we decribe the effects of the mammalian A-9 desaturase on fatty acid composition of membrane and storage lipids. 

Keywords— Transgenic brinjal. Bacillus thuringiensis, crylAb, Agrobacterium transformation, Meloidogyne incognita... 

Guri A, Sink K C (1988) Agrobacterium transformation of eggplant J. Plant Physiol. 133 52-55... 

Cheng X Y, Sardana R, Kaplan H et al. (1998) Agrobacterium-transformed rice plants expressing synthetic crylA(b) and crylA(c) genes are highly toxic to striped stem borer and yellow stem borer. Proc. Natl. Acad. Sci. USA. 95 2767-2772... 

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