Tissue-specific targeting

We have previously developed an in vivo selection method in which peptides that home to specific vascular beds are selected after intravenous administration of a phage display random peptide library [5]. This strategy revealed a vascular address system that allows tissue-specific targeting of normal blood vessels [6-8] and angiogenesis-related targeting of tumor blood vessels [3, 6, 9-12]. While the biologi-... 

Tissue-specific targeting of antigens can greatly facilitate the harvesting of proteins. Streaffield et al. [28] expressed antigens specifically in maize kernels, Sandhu et al. 

N. Kasahara, A. M. Dozy, and Y. W. Kan, Tissue-specific targeting of retroviral vectors through ligand-receptor interactions, Science 266 1373 (1994). 

This technique represents an innovative method to isolate antibodies against novel membrane molecules and epitopes expressed in their native conformation and allows the identification of new tissue-specific targeting molecules. From this point of view, this approach opens a new perspective in phage display technology and represents the first key step toward in vivo application. 

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