Tandem mass spectrometry multiple stages

Micro Raman spectroscopy Mass spectrometry Multiple-stage mass spectrometry tandem mass spectrometry... 

Waridel P, Wolfender J-L, Ndjoko K, Hobby KR, Major HJ and Hostettmann K. 2001. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. J Chromatogr A 926(1) 29-41. 

Selected Reaction Monitoring (SRM) Data acquired from specific product ions corresponding to m/z selected precursor ions recorded via two or more stages of mass spectrometry. Selected reaction monitoring can be preformed as tandem mass spectrometry in time or tandem mass spectrometry in space. The term multiple reaction monitoring is deprecated [1],... 

MS" NIH NIST multiple stages of tandem mass spectrometry National Institute of Health (USA) National Institute of Standards and Technology (USA)... 

Cao P. and Stults J.T. (2000), Mapping the phosphorylation sites of proteins using on-line immobilized metal-ion affinity chromatography/capillary electrophore-sis/electrospray ionization multiple stage tandem mass spectrometry, Rapid Commun. Mass Spectrom. 14(17), 1600-1606. 

Tandem mass spectrometry, more commonly known as MS-MS, utilises multiple stages of analysis. It is very selective and provides useful structural information. A variety of scan modes can be used to provide specific information for structural... 

Carte, N., Cavusoglu, N., Leize, E., Van Dorsselaer, A., Charlet, M and Bulet, P. (2001) De novo sequencing by nano-electrospray multiple-stage tandem mass spectrometry of an immune-induced peptide of Drosophila melanogaster. Eur. J. Mass Spectrom. 7(4), 399 108. 

Y. Wada, M. Tajiri, and S. Yoshida, Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics, Anal. Chem., 76 (2004) 6560-6565. 

The ability to apply customized waveforms to the QIT, together with the fact that ions are trapped and therefore can be subjected to different treatment over a period of time, makes the QIT one of the most versatile of mass spectrometers, rivaled only by the ICR mass spectrometer. This is most strongly evident in tandem mass spectrometry (MS/MS and related techniques), which will be discussed separately. It should be noted here, however, that multiple stage MS/MS experiments (MS/MS/MS.. ., or MS ) experiments are readily performed in ion traps. 

Sixty saponins, differing in carbohydrate substructures Glycosylated triterpenes Tree Quillaja saponaria var. Molina Preparative RPC UV at 206 nm ESI ion trap multiple stage tandem mass spectrometry 152... 

Waridel, P Wolfender, J.-L. Ndjoko, K. Hobby, K.R. Major, H.J. Hostettmann, K. Evaluation of Quadrupole Time-of-Flight Tandem Mass Spectrometry and Ion-Trap Multiple-Stage Mass Spectrometry for the Differentiation of C-glycosidic Flavonoid Isomers, J. Chromatogr. A 926(1), 29 1 (2001). 

Van Setten DC, Zomer G, Van DeWerken G, Wiertz EJHJ, Leeflang BR, Kamerling JP (2000) Ion Trap Multiple-Stage Tandem Mass Spectrometry as a Pre-NMR Tool in the Structure Elucidation of Saponins. Phytochem Anal 11 190... 

Song FR, Cui M, Liu ZQ, Yu B, Liu SY (2004) Multiple-Stage Tandem Mass Spectrometry for Differentiation of Isomeric Saponins. Rapid Commun Mass Spectrom 18 2241... 

Tandem mass spectrometry (MS/MS), in its simplest and earliest implementations on quadrupole ion traps (QlTs), employed sequential (tandem-in-time MS/MS) isolation and energetic (for example, collisional) activation steps [16-22]. Modem MS/ MS experiments can involve multiple stages of mass selectivity (MS"), which employ different dissociation methods. Depending upon precursor chemistry, sometimes... 

It has been demonstrated also that the iTRAQ tandem mass spectrometric quantitative analysis strategy can be used in conjunction with the quadrupole ion trap by performing multiple stages of mass analysis (that is, MS ) [125], For example, chemical derivatization with the iTRAQ reagent not only labels the N-terminus of a peptide, but the lysine side chain also. Thus, tryptic peptides with a modified lysine residue present at the C-terminus will produce a yj product ion at m/z 291 following ClD-tandem mass spectrometry. To generate the low m/z iTRAQ reporter ions required for quantitation, the yj product ion is isolated and subjected to data-dependent CID-MS. Using this approach, peptide identification is achieved in the MS/MS scan, while quantitation is achieved via MS. ... 

Hsu, F.-F., Turk, J. (2005) Studies on phosphatidylserine by tandem quadrupole and multiple stage quadrupole ion-trap mass spectrometry with electrospray ionization structural characterization and the fi agmen-tation processes./Aw Soc Mass Spectrom, 16, 1510-1522. 

Multiple detections, such as HPLC-UV-MS", HPLC-UV-NMR, HPLC-DAD-FD-UV, and HPLC-DAD-FD-MS, have been adopted to identify and characterize the structure of flavonoids and also used to evaluate the bioactivity of components [72, 84—87]. In most cases, single-stage MS is used in combination with U V detection to facilitate the confirmation of the identity of flavonoids in a sample with the help of standards and reference data (an example is given in Fig. 69.7, [88]). For the identification of unknowns, tandem mass spectrometry is used. 

Song F, Cui M, Liu Z, Yu B, Liu S. Multiple-stage tandem mass spectrometry for differentiation of isomeric saponins. Rapid Commun Mass Spectrom. 2004 18 2241-8. 

This evidence clearly shows that, at least in the simple case of GlyGlyGlyGly, water-loss products are not predominantly composed of an oxazolone structure caused by the loss of water from the C-terminus, but instead are composed of a structure formed by loss of water from across peptide bonds of the peptide s backbone, in this case probably an imidazolidinone. While this specific fragmentation pathway should not obscure MS/MS sequencing experiments, it is another potential source of confusion for further (e.g., MS ) stages of tandem mass spectrometry. Furthermore, this example underscores the fact that in most instances there are multiple potential fragmentation pathways in competition. 

Glish, G.L. Multiple Stage Mass Spectrometry the Next Generation Tandem Mass Spectrometry Experiment. Analyst 1994,119, 533-537. 

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