Tandem mass spectrometry stages

The main advantages of the ms/ms systems are related to the sensitivity and selectivity they provide. Two mass analyzers in tandem significantly enhance selectivity. Thus samples in very complex matrices can be characterized quickly with Htde or no sample clean-up. Direct introduction of samples such as coca leaves or urine into an ms or even a gc/lc/ms system requires a clean-up step that is not needed in tandem mass spectrometry (28,29). Adding the sensitivity of the electron multiplier to this type of selectivity makes ms/ms a powerhil analytical tool, indeed. It should be noted that introduction of very complex materials increases the frequency of ion source cleaning compared to single-stage instmments where sample clean-up is done first. 

Tandem mass spectrometry (MS-MS) is a term which covers a number of techniqnes in which one stage of mass spectrometry, not necessarily the first, is used to isolate an ion of interest and a second stage is then nsed to probe the relationship of this ion with others from which it may have been generated or which it may generate on decomposition. The two stages of mass spectrometry are related in specific ways in order to provide the desired analytical information. There are a large nnmber of different MS-MS experiments that can be carried ont [9, 10] bnt the fonr most widely nsed are (i) the prodnct-ion scan, (ii) the precnrsor-ion scan, (iii) the constant-nentral-loss scan, and (iv) selected decomposition monitoring. 

MS-MS A number of techniques in which two stages of mass spectrometry are used in series to probe the relationship between ions formed from an analyte - also known as tandem mass spectrometry. 

MS11 Multiple-stage mass spectrometry tandem mass spectrometry... 

Waridel P, Wolfender J-L, Ndjoko K, Hobby KR, Major HJ and Hostettmann K. 2001. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. J Chromatogr A 926(1) 29-41. 

Selected Reaction Monitoring (SRM) Data acquired from specific product ions corresponding to m/z selected precursor ions recorded via two or more stages of mass spectrometry. Selected reaction monitoring can be preformed as tandem mass spectrometry in time or tandem mass spectrometry in space. The term multiple reaction monitoring is deprecated [1],... 

MS" NIH NIST multiple stages of tandem mass spectrometry National Institute of Health (USA) National Institute of Standards and Technology (USA)... 

Cao P. and Stults J.T. (2000), Mapping the phosphorylation sites of proteins using on-line immobilized metal-ion affinity chromatography/capillary electrophore-sis/electrospray ionization multiple stage tandem mass spectrometry, Rapid Commun. Mass Spectrom. 14(17), 1600-1606. 

Note Tandem mass spectrometry is also known as MS/MS or MS. The latter terminology elegantly allows for the expansion to multi-stage experimental setups, e.g., MS MS or generally MS . 

Tandem mass spectrometry, more commonly known as MS-MS, utilises multiple stages of analysis. It is very selective and provides useful structural information. A variety of scan modes can be used to provide specific information for structural... 

Carte, N., Cavusoglu, N., Leize, E., Van Dorsselaer, A., Charlet, M and Bulet, P. (2001) De novo sequencing by nano-electrospray multiple-stage tandem mass spectrometry of an immune-induced peptide of Drosophila melanogaster. Eur. J. Mass Spectrom. 7(4), 399 108. 

Tandem mass spectrometry, abbreviated MS/MS, is any general method involving at least two stages of mass analysis, either in conjunction with a dissociation process or in a chemical reaction that causes a change in the mass or charge of an ion [1-3]. 

Y. Wada, M. Tajiri, and S. Yoshida, Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics, Anal. Chem., 76 (2004) 6560-6565. 

F. Kjeldsen, K. F. Haselmann, B. A. Budnik, E. S. Sorensen, and R. A. Zubarev, Complete characterization of posttranslational modification sites in the bovine milk protein PP3 by tandem mass spectrometry with electron capture dissociation as the last stage, Anal. Chem., 75 (2003) 2355-2361. 

Tandem mass spectrometry (MS/MS) Involves three main stages (i) selection of parent (precursor) ion (ii) fragmentation of parent ion hy so-called collision-induced dissociation (CID) and (iii) analysis of fragmented daughter (product) ions. MS/MS uses mass analysers in series, hence the name tandem mass spectrometry. 

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