T protein

Pawson, T. Protein modules and signaling networks. Nature 373 573-580, 1995. 

Cohn, E. J., and Edsall, J. T., Proteins, Amino Acids, and Peptides as Ions and Dipolar Ions, ... 

For the most part, plastics are man-made since very few plcistlcs are natural, i.e.- nature-made. Natural plastics include large molecular-wei t proteins and similar molecules. Man-made plastics can be classified as either thermoplastic or thermosetting. Each class derives its physical properties from the effects of application of heat, the former becoming "plastic" (that is- it becomes soft and tends to flow) while the latter becomes less "plastic" and tends to remain in a softened state. This difference in change of state derives from the actual nature of the chemical bonds in the polymer. Thermoplastic polymers generally consist of molecules composed of many monomeric units. A good example is that of polyethylene where the monomeric unit is -(CH2-CH2)-. The molecule is linear... 

Giglione, C. Boularot, A. Meinnel, T. Protein AMerminal methionine excision. Cell. Mol. Life Sci. 2004,61,1455-1474. 

Zeng, Jun Fridman, M. Maruta, FI. Treutlein, Flerbert R. Simonson, T., Protein-protein recognition an experimental and computational study of the R89K mutation in Raf and its effect on Ras binding, Prot. Set 1999, 8, 50-64. 

Hunter, T. Protein kinases and phosphatases the yin and yang of protein phosphorylation and signaling. Cell 80 225-236,1995. 

Fig. 1. (opposite page) Distribution of FITC-conjugated BSA in various fibroblast cell lines under different fixation/permeabilization regimes. (A-D) Protein distribution in living cells (A) PtKj, (B) CHO, (C) 3T3, and (D) HeLa cells. The protein is excluded from the nuclei of all cells. (E-H) Protein distribution in cells extracted for 10 min with 0.1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (E) PtKi, (F) CHO, (G) 3T3, and (H) HeLa cells. Nuclear fluorescence is seen in (E) PtKj and (G) 3T3 cells. (I-L) Protein distribution in cells extracted for 10 min with 1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (I) PtKj, (J) CHO, (K) 3T3, and (L) HeLa cells. No fluorescence is detected in the cells with the exception of some nuclear fluorescence seen in (L) HeLa cells. (M-P) Protein distribution in cells fixed for 30 min with 3.7% paraformaldehyde before permeabilization for 10 min with 0.1% Triton X-100. Fluorescence is seen primarily in the cytoplasm with the exception that nuclear fluorescence is seen in (M) PtKi and (N) CHO cells. (Q-T) Protein distributions in cells fixed for 5 min with 90% methanol, 50 vaM EGTA at -20°C (Q) PtKj, (R) CHO, (S) 3T3, and (T) HeLa cells. All cells show an overall low fluorescence, fibrous-textured cytoplasmic fluorescence, and bright staining at the periphery of the nucleus. 10 mm per scale division (black bar). (Reproduced with permission from ref. 6.)... 

Kodadek, T., Protein microarrays prospects and problems, Chem. Biol., 8, 105-115, 2001. 

IV then based on response 250 g/48 h max Peds. 0.5—1 g/kg/dose inf at 0.05-0.1 g/min Caution [C, ] Sev e anemia cardiac, renal, or h atic insuff d/t protein load h5 pCTVolemia Contra CHF Disp Soln SE Chills, fevCT, CHF, tach, -1- BP, hypervolemia Interactions At5 pical Rxns W/ ACEI w/hold 24 h prior to plasma administration EMS May cause pulm edema, monitor resp status/lung sounds OD May cause circulatory ov load (T venous pressure) or pulm edema slow flow to KVO and evaluate... 

Sobek, O., Adam, R, Taborsky, L., Zeman, D., Kelbich, P., and Hildebrand, T., Protein fractions and other parameters of cerebrospinal fluid in patients with multiple sclerosis. Clin. Biochem. Metab. 8(29), 209-212 (2000). 

Harris, K. W., and Esmon, C. T, Protein S required for bovine platelets to support activated protein C binding and activity. J. Biol. Chem. 260, 2007—2010 (1985). 

Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yohida T. Protein and polymer analyses up to mjz 100,000 by laser ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 1988 2 151-153. 

Fig. 2.1. Semilogarithmic plot of molecular weight (Mr) of marker proteins vs relative mobility (Rf) of marker proteins in gels of different acrylamide concentrations %T. Proteins 1 aprotinin (6.5 kD) 2 lysozyme (14.5 kD) 3 soybean trypsin inhibitor (21.5 kD) 4 carbonic acid anhydrase (31 kD) 5 hen ovalbumin (45 kD) 6 bovine serum albumin (66 kD) 7 phosphorylase b (97.4 kD) 8 8-galactosidase (116 kD) 9 myosin (205 kD)...

Hunter, T. Protein kinase classification (1991) Methods in Enzym. 200, 3-37... 

Oancea, E. and Meyer, T. Protein kinase C as a molecular machine for decoding calcium and dia-cylglycerol signals (1998) CeU 95, 307-318... 

Pawson, T. Proteine modules and signalling networks (1995) Nature 373, 573-579... 

Rassoulzadegan, M., Naghashfar, Z., Cowie, a., Carr, A., Grisoni, M., Kamen, R., and CUZIN, F. (1983). Expression of the large T protein of polyoma virus promotes establishment in culture of normal rodent fibroblast cell lines, Proc. NatL Acad. Sci. 80, 4354. 

A sheep blastocyst library was screened with a probe based on the N-terminal sequence of the IFN-T protein and the cDNA obtained (Table 3). Surprisingly, it exhibited 45-55% homology with various IFNs from human, mouse, rat, and pig and 70% homology with bovine IFN-co [12]. It shared both molecular weight (19 kDa) and pi (5.4-5.6) with IFN-as, while its length, 172 amino acids, was equivalent to the IFN-cos. In competition studies, IFN-T was found to compete with IFNs a, (3, and for binding to the Type IIFN receptor [13]. In contrast, IFN-T exhibited several unique properties such as its reproductive function, its poor inducibility by virus, and its apparent reduced cytotoxicity. Thus, IFN- conceptus protein appears to be a novel IFN. 

Martinez, W. H. Hopkins, D. T. "Protein Nutritional Quality of Foods and Feeds" Friedman, M., Ed. Marcel Dekker Inc. New York, 1975. 

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