Syrian hamster melanoma

Origins of parental lines Ri and Rj-s mass population and a clone, derived from whole Wistar rat embryo (Microbiological Associates). Recl-C clone derived from Recl-1, a diploid rat embryo strain (Petursson et al., 1964). NH line derived from the Novikoff hepatoma by Dr. E. Swim (unpublished). BiJu BUDR-resistant line of Chinese hamster fibroblasts (Humphrey and Hsu, 1965). 3460-3 8-azaguanine resistant clone of a Syrian hamster melanoma (Moore, 1964). Cl 1 D, B 82, and A 9 BUDR or 8-azaguanine resistant sublines of L cells (Kit et al., 1963 Littlefield, 1964). 2472-6 and 2472-6-3 clone and 8-azaguanine resistant subclone of Sanford s high cancer line (Sanford et al., 1954). 2555 Sanford s low cancer line (Sanford et al., 1954). 

As can be seen in Table 2, hybrids between cells of a Syrian hamster melanoma line and aneuploid mouse cells of Sanford s high cancer line are listed as asynchronous although, at 29 C, numerous hybrid mitoses are observed 2 days after the initiation of mixed cultures, in up to 50% of these metaphases, the chromosomes of the two species appear to be in very different stages of contraction or coiling [this is so whether colchicine is used or not and whedier, prior to karyological analysis, the cultures are incubated for 5 hours at 29°... 

However, when Syrian (golden) hamster melanoma cells were fused with non-melanin-producing C3H mouse cells [104], and the hybrid line was isolated by the appropriate selection, no melanin production was observed in the hybrid cells, nor were tyrosinase and dopa oxidase activities detected in their extracts [104]. The parental melanoma cells had the enzymic activities and produced pigment. Nevertheless, in the hybrid cells, which possessed the expected hybrid karyotype, the lactate and malate dehydrogenase isozyme patterns were combinations of both parental types [104]. Thus, one might conclude that in the non-melanin-producing C3H parental cells there existed a specific diffusible enzyme... 

Syrian golden hamsters bearing Greene melanoma The modulative effect of 3AB on the progression of Greene melanomas in Syrian golden hamsters after treatment with X-rays or DTIC was studied. This tumor was successfully retransplanted more than 20 times before our... 

Fig. 1. Relation between tumor size and its volume in Syrian golden hamsters bearing Greene melanoma. 59 tumors were measured, dissected and weighed.

Fig. 2b. Relation between increased therapeutic effect of X-rays (4x4 Gy) and concentration of SAB in Syrian golden hamsters bearing Greene melanoma.

Fig. 3. (left) Reduced tumor progression by fi actionated dose of X-rays of 32 Gy (4 x 8 Gy with 24 hr intervals between fractions) in the presence or absence of 3AB in Syrian golden hamsters bearing Greene melanoma. 

In the present study we found that 3AB alone has no therapeutic effect and no influence on the growth kinetics of untreated Greene melanoma or survival of untreated leukemic mice. X-rays and DTIC treatment alone could induce growth delay of the melanotic tumors in Syrian golden hamsters. Additional treatment with 3AB resulted not only in an increase in the flierapeutic effect of X-rays or DTIC, but also in the survival of DTIC treated hamsters and leukemic DBA2 mice. 

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