High cancer line

Origins of parental lines Ri and Rj-s mass population and a clone, derived from whole Wistar rat embryo (Microbiological Associates). Recl-C clone derived from Recl-1, a diploid rat embryo strain (Petursson et al., 1964). NH line derived from the Novikoff hepatoma by Dr. E. Swim (unpublished). BiJu BUDR-resistant line of Chinese hamster fibroblasts (Humphrey and Hsu, 1965). 3460-3 8-azaguanine resistant clone of a Syrian hamster melanoma (Moore, 1964). Cl 1 D, B 82, and A 9 BUDR or 8-azaguanine resistant sublines of L cells (Kit et al., 1963 Littlefield, 1964). 2472-6 and 2472-6-3 clone and 8-azaguanine resistant subclone of Sanford s high cancer line (Sanford et al., 1954). 2555 Sanford s low cancer line (Sanford et al., 1954). 

As can be seen in Table 2, hybrids between cells of a Syrian hamster melanoma line and aneuploid mouse cells of Sanford s high cancer line are listed as asynchronous although, at 29 C, numerous hybrid mitoses are observed 2 days after the initiation of mixed cultures, in up to 50% of these metaphases, the chromosomes of the two species appear to be in very different stages of contraction or coiling [this is so whether colchicine is used or not and whedier, prior to karyological analysis, the cultures are incubated for 5 hours at 29°... 

The monolayer culture technique is frequently employed in cytotoxicity tests with cancer lines and in studies of chemical sensitivity of different tumor types. This method offers high flexibility with respect to drug exposure and recuperation conditions, as well as to the quantification of any effect. Among all methods, cell culture in a monolayer requires a lower cell number and allows the evaluation of multiple drugs in large concentration ranges. 

The ability of PGA to inhibit the growth of ovarian cancer cells was examined in vitro, in the presence and absence of DON. The MAWS cell line is a primary ovarian cancer cell line established from a human patient. As documented in Table 6.2, this cell line is highly sensitive to PGA alone, even at low concentrations, but when PGA was combined with DON the growth inhibition was almost complete. Other established ovarian cancer lines such as OVGAR3 were also found to be sensitive both to PGA alone and PGA-t DON therapy (Table 6.2 Fig. 6.1). 

The role of NSAIDs in MSI (microsatellite iustability)-high colon cancers has been less clear. MSI-high cancers typically have less expression of putative NSAID targets such as COX-2 and AKT." A number of studies have shown that NSAIDs can induce apoptosis in MSI-high colon cancer cell lines, potentially through induction of pro-apoptotic MMR (mismatch repair) proteins. ... 

Fig. 7.3. Transfer of risk in a population with low to a population with high cancer rates leftpanel) and transfer of risk in a population with high to a population with low cancer rates right panel). The broken lines in the upper panels are the increased cancer rates representing a relative excess of 50%. The transfer of the excess relative rate of 0.5 ( multiplicative model ) to a population with diverging cancer rates solid lines below) results in the dotted lines in the lower panels. A transfer of the excess absolute rate ( additive model ), Le., the difference between the solid and the broken lines in the upper panelsy results in the dashed lines in lower panels...

Plakortides M and N, recently isolated from Plakortis halichondrioides harvested in Puerto Rico, are highly cytotoxic in vitro for several cancer lines, with an IC50 value that is often below 1 jxM (Jimenez, Garzon, and Rodriguez, 2003). 

These last two derivatives are highly cytotoxic to the cancer line A431 (squamous cell carcinoma SCC), vrith an IC50 of about 17ngml (Ojika et al., 2003). 

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