Surgical pathology

Biopsy material for amebiasis should be processed for surgical pathology and then examined for ulcers containing amebae. The periodic acid-Schiff stain counterstained with hematoxylin is particularly helpful because amebae stain more positively with periodic acid-Schiff stain than do inflammatory cells, and amebae show typical amebic nuclei. Of course, there are no amebic cysts in tissue. 

The advent of personalized therapies that are dependent on the outcome of an immunohistochemical stain has increased the need for quantitative positive controls. When IHC was first introduced as an adjunct in surgical pathology diagnosis, the interpretation was largely qualitative. Specific markers were present or absent, thereby characterizing a tumor cell s lineage. The fact that IHC interpretation was qualitative, rather than semiquantitative, minimized... 

Taylor CR, Kledzik G. Immunohistologic techniques in surgical pathology—a spectrum of new special stains. Hum. Pathol. 1981 12 590-596. 

Banks, PM. Technical aspects of specimen preparation and special studies. In Surgical Pathology of the Lymph Nodes and Related Organs, ed. ES Jaffe, pp. 1-21. Philadelphia W. B. Saunders, 1985. 

However, ICC is not used as frequently in cytology as IHC in surgical pathology due to some technical issues, such as scanty or lack of diagnostic material in cell blocks or cytospins, and questionable reliability of immunos-taining on smears. 

David G. Hicks, Professor and Director, Surgical Pathology Unit, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY... 

Pinkus, G. S. and Kurtin, P. J. (1985) Epithelial membrane antigen—A diagnostic discriminant in surgical pathology immunohistochemical profile in epithelial, mesenchymal, and hematopoietic neoplasms using paraffin sections and monoclonal antibodies. Hum. Pathol. 16, 929-940. 

Surgical Pathology of Lung Neoplasms, edited by A. M. Marchevsky... 

Morales AR, Nassiri M, Kanhoush R, et al. Experience with an automated microwave assisted rapid tissue processing method Effect on histology and timeliness of diagnostic surgical pathology. Am. J. Clin. Path. 2004 121 528-36. 

Taylor CR, Cote RJ. Immunomicroscopy, A Tool for the Surgical Pathology, 3rd Edition, Edinburgh Elsevier, 2005... 

The consensus arising from these meetings was that the translation of IHC methods from bench to bedside , in the sense of use in routine surgical pathology, has been greatly hindered by two key factors. While decades have passed these issues have not been satisfactorily addressed and have not been ameliorated. 

The successful application of immunohistochemical methods to formalin-fixed surgical pathology specimens stimulated rapid progress in this newly emerging field, and in quick succession came the introduction of the immunoperoxidase bridge method (2) and the peroxidase anti-peroxidase (PAP) complex method (3). 

Tissues and cells in situ end-labeling techniques can be applied to cells grown in culture, to frozen tissue sections, or to formalin-fixed, paraffin-embedded material, such as that found in surgical pathology archives. 

An effective tuberculosis control plan will include determination of exposure at regular intervals for all employees who are at occupational risk. Engineering and work practice controls are particularly important in laboratory areas, such as surgical pathology and microbiology. But there is clearly a risk of exposure from specimens of patients with suspected or confirmed tuberculosis in every section of the laboratory, including chemistry. 

Davies, M.P., Sibson, R., West, C.R., Smith, P.H. and Foster, C.S. (2003) Declining estrogen receptor-p expression defines malignant progression of human breast neoplasia. The American Journal of Surgical Pathology, 27, 1502-1512. 

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