Steeped corn

Figure 9.2 Section of steeped corn horny endosperm (1 Op,m thick, iodine stained, magnified X612 SG, starch granules PM, protein matrix CW, cell wall).

Figure 9.13 Battery of hydroclones used for separation of germ from milled, steeped corn. (Courtesy of Dorr-Oliver, Inc., Stamford, Connecticut)...

Corny Aromatic characteristic of steeped corn and Crude corn oil diluted 5 95 in good quality... 

Table XXIII. Typical Results of Fractionation by Laboratory Wet Milling of Steeped Corn...

Water, steep corn Steep acid, acidity at end of steeping cycle as hydrochloric acid 0.56%, sulfur dioxide initially 0.12%, finally 0.012%, pH 4.1 average (below corn level) Corn Products F 130 31 XX XX <00001 W0.0013C <0.0001 W0.0012 <0 0001 ... 

Starch is insoluble in cold water, but in hot water the granules gelatinize to form an opalescent dispersion. It is made from corn, wheat, potatoes, rice and other cereals by various physical processes such as steeping, milling and sedimentation. It is used as an adhesive, for sizing paper and cloth, as an inert diluent in foods and drugs, and for many other purposes. 

Corn starch [9005-25-6] Corn steep liquor Corn stover Corn sugar Corn sweetener Corn sweeteners Corn syrup... 

The following process description is taken from U.S. Patent 2,987,449. An appropriate S. aureofaciens strain such as mutant S1308 (ATCC No. 12,748) is grown aerobically in a suitable inoculum medium. A typical medium used to grow the primary inoculum is prepared according to the following formula sucrose, 20.0 g corn steep liquor, 16.5 ml ammonium sulfate, 2.0 g calcium carbonate, 7.0 g and water to 1,000 ml. 

Soybean meal Cornstarch Corn steep liquor Sodium nitrate... 

Corn starch, g/l CaCOs, g/l (NH4)jS04, g/l NH4CI, g/l FeS04 7H20, mg/l IVInS04 4H20, mg/l ZnS04-7H2 0, mg/l CoCIj OHjO, mg/l Corn steep liquor, g/l Cottonseed meal, g/l Lard oil, % v/v... 

An inoculum broth is prepared having the following composition 32 pounds starch 32 pounds soybean meal 10 pounds corn steep solids 10 pounds sodium chloride 6 pounds calcium carbonate and 250 gallons water. 

In a 1,600-gallon iron tank is placed a fermentation broth having the following composition 153 pounds starch 153 pounds soybean meal 51 pounds corn steep solids 33 pounds calcium carbonate 51 pounds sodium chloride and 1,200 gallons water. 

Bacterium PenicHHum patulum Corn steep liquor... 

Fe" (2 ppm), casein hydrolyzate (0.2 g/dl), yeast extract (0.2 g/dl), corn steep liquor (0.2 ml/dl), polypeptone (0.1 g/dl), meat extract (0.1 g/dl) and sodium ribonucleate (10 mg/dl) were poured into respective test tubes and each tube was sterilized at 115°C for 10 minutes. Thereafter separately sterilized calcium carbonate was added in the amount of 2 g/dl and then cells of Bacillus subtUis S26910 were Inoculated into the above media and cultured with shaking at 30°C for 20 hours. 

The yield was highest with starch or dextrin, intermediate and about the same with sucrose, glucose, maltose and lactose and poorest with glycerol. Kanamycin was produced by media containing soybean meal, peanut meal, cottonseed meal, corn steep liquor, peptone, yeast extract or meat extract, with or without sodium nitrate. Commercially available soybean meal was recognized to be one of the best nitrogen sources. The addition of corn steep liquor, peptone, yeast extract or nitrate to the soybean meal promoted the production of kanamycin. 

The following process description is taken from U.S. Patent 2,897,218. Six 100-ml portions of a medium in 250-ml Erlenmeyer flasks containing 1% glucose, 2% corn steep liquor (60% solids) and tap water was adjusted to a pH of 4.9. This medium was sterilized for 45 minutes at 15 psi pressure and inoculated with a one to two day growth of Septomyxa affinis ATCC 6737. The Erlenmeyer flasks were shaken at room temperature at about 24°C for a period of 3 days. 

At the end of this period, this 600-ml volume was used as an inoculum for ten liters of the same glucose-corn steep liquor medium which in addition contained 10 ml of an antifoam (a mixture of lard oil and octadecanol). The fermentor was placed into the water bath, adjusted to 28°C, and the contents stirred (300 rpm) and aerated (0.5 liter air/10 liters beer). After 17 hours of incubation, when a good growth developed and the acidity rose to pH 6.7, 2 g of 6a-methylhydrocortisone plus 1 g of 3-ketobisnor-4-cholen-22-al, dissolved in 115 ml of dimethylformamide, was added and the incubation (conversion) carried out at the same temperature and aeration for 24 hours (final pH 7.9). 

The Fermentation Process The process by which this antifungal substance is produced is an aerobic fermentation of an aquaous nutrient medium inoculated with a pimaricin-producing strain of Streptomycesgihrosporeus. The nutrient medium contains an assimilable source of carbon such as starch, molasses, or glycerol, an assimilable source of nitrogen such as corn steep liquor and Inorganic cations such as potassium, sodium or calcium, and anions such as sulfate, phosphate or chloride. Trace elements such as boron, molybdenum or copper are supplied as needed in the form of impurities by the other constituents of the medium. 

Nitrogen sources include proteins, such as casein, zein, lactalbumin protein hydrolyzates such proteoses, peptones, peptides, and commercially available materials, such as N-Z Amine which is understood to be a casein hydrolyzate also corn steep liquor, soybean meal, gluten, cottonseed meal, fish meal, meat extracts, stick liquor, liver cake, yeast extracts and distillers solubles amino acids, urea, ammonium and nitrate salts. Such inorganic elements as sodium, potassium, calcium and magnesium and chlorides, sulfates, phosphates and combinations of these anions and cations in the form of mineral salts may be advantageously used in the fermentation. 

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