Corn steep water

Cereal grains are particularly rich in phytates corn steep water produced in the wet milling of corn, is one of the best sources of such material. To recover the phytate from corn steep water it is customary to neutralize the same with... 

The following process description is taken from U.S. Patent 2,987,449. An appropriate S. aureofaciens strain such as mutant S1308 (ATCC No. 12,748) is grown aerobically in a suitable inoculum medium. A typical medium used to grow the primary inoculum is prepared according to the following formula sucrose, 20.0 g corn steep liquor, 16.5 ml ammonium sulfate, 2.0 g calcium carbonate, 7.0 g and water to 1,000 ml. 

An inoculum broth is prepared having the following composition 32 pounds starch 32 pounds soybean meal 10 pounds corn steep solids 10 pounds sodium chloride 6 pounds calcium carbonate and 250 gallons water. 

In a 1,600-gallon iron tank is placed a fermentation broth having the following composition 153 pounds starch 153 pounds soybean meal 51 pounds corn steep solids 33 pounds calcium carbonate 51 pounds sodium chloride and 1,200 gallons water. 

The following process description is taken from U.S. Patent 2,897,218. Six 100-ml portions of a medium in 250-ml Erlenmeyer flasks containing 1% glucose, 2% corn steep liquor (60% solids) and tap water was adjusted to a pH of 4.9. This medium was sterilized for 45 minutes at 15 psi pressure and inoculated with a one to two day growth of Septomyxa affinis ATCC 6737. The Erlenmeyer flasks were shaken at room temperature at about 24°C for a period of 3 days. 

At the end of this period, this 600-ml volume was used as an inoculum for ten liters of the same glucose-corn steep liquor medium which in addition contained 10 ml of an antifoam (a mixture of lard oil and octadecanol). The fermentor was placed into the water bath, adjusted to 28°C, and the contents stirred (300 rpm) and aerated (0.5 liter air/10 liters beer). After 17 hours of incubation, when a good growth developed and the acidity rose to pH 6.7, 2 g of 6a-methylhydrocortisone plus 1 g of 3-ketobisnor-4-cholen-22-al, dissolved in 115 ml of dimethylformamide, was added and the incubation (conversion) carried out at the same temperature and aeration for 24 hours (final pH 7.9). 

Corn steep liquor contains 2.5% invert sugars and 50% water. The rest of the feed is considered as residual solids. Beet molasses containing 50% sucrose, 1% invert sugar, 18% water and remainder solids are mixed with corn steep liquor in a mixing tank. Water is added to produce a diluted mixture with 2% invert sugar, 125 kg com steep hquor and 45 kg molasses, which is fed into an enzymatic hydrolysis tank. 

A medium (containing corn steep liquor calcium carbonate sucrose ammonium, ferrous, manganese, and zinc sulfates and ammonium, cobalt, and magnesium chlorides) is sterilized and diluted with water to the desired concentration. It is inoculated with Streptomyces aureofaciens, kept at 27°C, and aerated and agitated for 60 hours, with lard oil added to control foaming (66). 

Cells of Nocardia autotrophica subsp. amethystina FERM P-6183 was inoculated from a slant culture by means of a platinum loop into each of twenty 500 ml Erlenmeyer flasks, each containing 100 ml of a culture medium having the following composition glucose - 1.0%, peptone - 0.2%, meat extract - 0.1%, yeast extract - 0.1%, corn steep liquor 0.3%, tap water balance.. 

The fungus Helirastylum piriforme ATCC 8992 is cultivated for 3 d at 28 "C in a medium (pH 6.8-7.0) consisting of 3.5 % glucose. 2% peptone and 0.3 % corn steep liquoT in tap water, on a rotary shaker. The wet mycelium harvested from the culture broth (100 ml) is suspended in 100 mL of deionized water and 50 mg (0.13 mmol) of finely divided lithocholic acid (3) are added. The mixture is incubated for 2 d at 28 JC on a shaker. Extraction of the incubation mixture with EtOAc yields a mixture of hydroxylation products. The principal component 4 is purified by column chromatography on silica gel and isolated by crystallization from EtOAc and then from acetone yield 20 mg ( 40%) mp 171.5- 172.5 C. 

Inositol. Inositol. I.2,3.S-rrprepared from natural sources such us corn steep liquors and is available in limited commercial quantities. It is a white crystalline powder that is soluble in water 1 6 and in dilute alcohol. It is. slightly soluble in alcohol, the usual organic solvents, and fixed oils. It is stable under normal storage conditions. 

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