Staining sudan black

Table 5.2.1 Reference values for lipoproteins stained with Sudan Black B. HDL High-density lipoprotein, LDL low-density lipoprotein, VLDL very-low-density lipoprotein...

The organic matrix of mollusc shells consists of soluble and insoluble fractions. The insoluble material is primarily hydrophobic. This is best demonstrated by the affinity of the matrix for lipoidal stains such as Sudan Black, even after thorough lipid extraction. The preponderance of aliphatic amino acids may explain this property413. ... 

Fig. 58. Star electrophoresis. Buffer, Veronal-sodium Veronalate, pH 8.6 i 0.06 substrate, Whatman 3 MM, dimensions, 30 x 30 cm electrical field, constant current 30 ma starting point, 16.1 volts/cm endpoint, 11.6 volts/cm sample, 0.02 ml serum, concentrated 5 times prestained with Sudan black duration, 3 hours, (a) Prestained for lipids, but before staining for protides, (b) After supplementary staining with amido black.

For a time, the question of the bacterial origin of these bodies was hotly debated. Hanks,208 from cytological evidence and the fact that such materials were confined to the leprosy bacillus and disappeared during sulfone therapy, persuasively reasoned that they originated in M. leprae. Moreover, since chloroform in aqueous systems declumped and dispersed M. leprae, he concluded that mycobacterial lipids were the major bonding substances in the electron-transparent material. Since the material of the capsule can be stained with Sudan Black B, Fisher and Barksdale209 and Nishiura et al.2 0 had concluded that the electron-transparent zone which surrounds M. leprae in vivo is lipid. 

The results indicate that Sudan black penetrated into the bulk phase of lens. The dye permeation test of LBL-coated lens in wet conditions did not show stain. However, when a thin layer of liquid water exists on the surface, the dye test losses validity in terms of evaluating the barrier property of the coating because the oil spreads on the water layer, which acts as a barrier to oil-soluble dye. Wet uncoated lens did show the dye stain, which probably indicates that water on uncoated lens is not as strongly held by the surface as LBL-coated surface. The dye permeation test of LBL-coated lenses under blotted and dried conditions showed stain. The stain in blotted sample was spotty and the color intensity is less than that for the dried surface, which is more intense than that for uncoated lens, i.e., dried LBL-coated lens has the strongest dye absorption. 

For LM and TEM, the treated apical meristems were fixed frozen in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.2 at 4°C overnight. Control unfrozen samples were also fixed in the same way. For TEM, tissues were postfixed in 1% OSO4 in water for 2 h, dehydrated in a graded ethanol-acetone series and embedded in low-viscosity Spurr s resin. Sections were stained with uranyl acetate followed by lead citrate and examined in a Zeiss EM109T transmission electron microscope. For LM, sections were stained with Sudan Black B (Sigma S 2380 Cl 26150) and Toluidine blue O (Sigma T 3260 Cl 52040). 

LM of sections of the axis showing the cortical parenchyma after staining with Sudan Black B. (a) Control (b) rapid cooling (c) slow cooling. Coalescence of lipid bodies can be observed (arrowheads). Abbreviations Protein vacuole (pv) starch (s). Bar = 20 p,m. 

Gorringe (G4) stains with lissamine green (BDH) using a solution containing 0.30 g dye in a mixture of 15.0 ml acetic acid and 100.0 ml distilled water. This stain can also be applied to films which have been previously stained with Sudan black, thus achieving a simultaneous coloration of proteins and lipids (W4). 

If precipitation lines contain a lipoid component, they can be stained with a combination of oil red and Sudan black (Ul), first using a solution of 1 g of oil red O or scarlet red R (Geigy) or Sudan IV dissolved in one liter of 60 % ethanol during 24 hours at 37 °G. The solution is filtered after cooling and kept in dark-colored bottles. The agar films remain 16 hours in this solution then they are soaked for 2 hours in a... 

Fig. 11. The presence of lipid substances in some Arabidopsis explant cells during the process of SE (Sudan black staining the arrows point to some of the cells with hpad lamellae in the wall bar = 10 pm author - Potocka).

Fig. 1. Bidimensional separation of HDL subfractions. Vertical Isoelectric focusing with the cathode at the bottom. Horizontal Left, whole plasma, followed by density subfractions from density ranges as assigned in the graph. The bands were first precipitated in the gel and then stained with Sudan black. It is apparent that isoelectric subfractions cumulate in differing density ranges...

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