Sudan black

Physical Form Dark brown powder Solubility Insoluble in water soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol 

Other Names IH-Perimidine, 2,3-dihydro-2,2-dimeth-yl-6-[[4-(phenylazo)-l-naphthalenyl]azo]- C.I. Solvent Black 3 Sudan Black B 2,3-Dihydro-2,2-dimethyl-6-[(4-phenylazo-l-naphthalenyl)-azo]-l//-perimidine 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-l-naphthyl] azojperimidine 6-(l-Phenylazo-4-naphthylazo)-2,3-dihy-dro-2,2-dimethylperimidine Acetylated Sudan Black B Aizen SOT Black 6 C.I. 26150 Ceres Black BN Chuo Sudan Black 141 Fast Black HBN Fat Black HB Fat Black HB 01 Hexatype Black B Lacquer Black S Lacquer Black VB NSC 11239 Neptune Black X 60 Nubian Black BT Oil Black 860 Oil Black BT Oil Black HBB Oil Black HZ Orient Oil Black 860 Orient Oil Black HBB SOT-Black 6 Solvent Black 3 Sudan Black 141 Sudan Black X 60 Sudan Deep Black BB Sudan Deep Black BN Typogen Black 

Merck Index Number 8885 Chemical/Dye Class Azo Molecular Formula C29H24N6 Molecular Weight 456.54 

Biological Applications Diagnosis of acute myeloid leukemia (AML) detecting neuron-specific nuclear protein NeuN drug screening  

Safety/Toxicity Acute oral toxicity, carcino- 

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Fig. 60 Chromatogram of a 6 dyestuff mixture made up (according to falling Rf values) of Sudan red 7B, Sudan orange G, Sudan black B, Sudan yellow, Artisil blue 2RP and Sudan black B (2 components) under different humidity conditions. From left to right 72, 65, 47, 42, 32,18, 14 and 9% relative humidity layer silica gel 60 mobile phase toluene.

Using quenching agents like Nile Blue and Sudan Black are effective in suppressing AF for confocal or widefield microscopy... 

Lipids Nile blue Sudan black B Sudan IV... 

Phospholipids Acid haematin Bromine—Sudan black Bromine—Acetone—Sudan black... 

Sectioning material for immunocytochemistry with glass and diamond knives is identical to the process used for conventional EM. One difficulty frequently encountered is that unosmicated tissue embedded in plastic is sufficiently cleared by the solvent and embedding process as to be almost invisible. This makes orientation of the tissue in the trimming of the blocks difficult and increases the chances of mistakes of cutting too much. A small amount of Sudan Black dye can be added to... 

Sudan Black B 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenyl)azo-lH-perimidine... 

Fig. 3.174. Example of NACE electropherogram of mixture of dyes (1, Janus Green 2, Brilliant Cresyl Blue 3, Sudan Black 4, Thymolphtalein 5, Phenolphtalein) in acetonitrile. Added ionic liquid was l-butyl-3-methyl fluoroacetate (3.3 mg/ml). Applied voltage +18kV, current 8 pA. Reprinted with permission from M. Vaher et al. [207].

Table 5.2.1 Reference values for lipoproteins stained with Sudan Black B. HDL High-density lipoprotein, LDL low-density lipoprotein, VLDL very-low-density lipoprotein...

The organic matrix of mollusc shells consists of soluble and insoluble fractions. The insoluble material is primarily hydrophobic. This is best demonstrated by the affinity of the matrix for lipoidal stains such as Sudan Black, even after thorough lipid extraction. The preponderance of aliphatic amino acids may explain this property413. ... 

Fig. 58. Star electrophoresis. Buffer, Veronal-sodium Veronalate, pH 8.6 i 0.06 substrate, Whatman 3 MM, dimensions, 30 x 30 cm electrical field, constant current 30 ma starting point, 16.1 volts/cm endpoint, 11.6 volts/cm sample, 0.02 ml serum, concentrated 5 times prestained with Sudan black duration, 3 hours, (a) Prestained for lipids, but before staining for protides, (b) After supplementary staining with amido black.

For a time, the question of the bacterial origin of these bodies was hotly debated. Hanks,208 from cytological evidence and the fact that such materials were confined to the leprosy bacillus and disappeared during sulfone therapy, persuasively reasoned that they originated in M. leprae. Moreover, since chloroform in aqueous systems declumped and dispersed M. leprae, he concluded that mycobacterial lipids were the major bonding substances in the electron-transparent material. Since the material of the capsule can be stained with Sudan Black B, Fisher and Barksdale209 and Nishiura et al.2 0 had concluded that the electron-transparent zone which surrounds M. leprae in vivo is lipid. 

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