Staining protection

Meanwhile, prepare the staining antibody cocktail. Mix APC-CD3, PE-CD25, and FITC-CD69 (10 pL each per tube to be stained). Protect the antibody cocktails from hght. (To investigate effects of inhibitors on specific T cell subsets, mix antibody cocktails accordingly.)... 

This fluid is for ferrous metals. The concentrate should be further diluted from 1 10 (heavy duty) to 1 40 (light duty) with water to form a ready-to-use fluid. For increased rust and stain protection, increase the amount of Mazon RI 4 A. For increased lubricity increase the amount of lubricant or extreme pressure additive. 

Diarylamiaes fuactioa as mbber antioxidants by breaking the peroxidative chain reactions leading to mbber deterioration. Nearly all commercial synthetic mbbers (see Elastomers, synthetic), including neoprene, butyl, styrene—butadiene, and the acrylonitrile—butadiene mbbers, can be protected with about 1—2% of an alkylated diphenylamine. DPA itself is not used as a mbber antioxidant. An objectionable feature of these antioxidants is that they cause discoloration and staining which limits their use to applications where this is not important. 

Protective Systems. As in the case of sulfur vulcani2ation, there have been many research workers in the field of natural mbber oxidation and its protection (39,40). The principal problem has always been to find an antioxidant that provides protection against flex-cracking, and yet does not stain. None has been found as of the mid-1990s, hence it is best to categori2e the various types according to their capabiUties. 

The color and effect produced by NGR stains and any stain mixture depend on several factors other than the colors or type of dyes used. Those factors include strength of the mixture, the amount appHed, the type of substrate, and the solvent system used for the stain. The role of the wood stain is not to provide protection rather, the primary function of the stain is to impart color effects by accentuating grain patterns. The transparency and brightness needed to enhance the natural beauty of the wood are optimized by using dye-type stains for wood. 

In the 3M Water/Alcohol Drop Test (41), this test is designed to provide a simple, rapid method to assess the aqueous stain resistance of substrates treated with a protective finish. 

Detection of Bromine Vapor. Bromine vapor in air can be monitored by using an oxidant monitor instmment that sounds an alarm when a certain level is reached. An oxidant monitor operates on an amperometric principle. The bromine oxidizes potassium iodide in solution, producing an electrical output by depolarizing one sensor electrode. Detector tubes, usefiil for determining the level of respiratory protection required, contain (9-toluidine that produces a yellow-orange stain when reacted with bromine. These tubes and sample pumps are available through safety supply companies (54). The usefiil concentration range is 0.2—30 ppm. 

The best oxidation inhibitors are not usually the best antio2onants (qv). A disubstituted i ra-phenylenediamine such as AJ-isopropyl-AT-phenyl- -phenylenediamine is often selected for that purpose. -Phenylenediamine derivatives iaterfere with cure chemistry and scorchiness, and can stain objects ia contact with the vulcani2ate (114). On balance, /V-(1,3-dimethy1buty1)-/V-phenyl- -phenylenediamine and phenyl /to1y1- -pheny1enediamines have the best combination of properties. They are less scorchy and provide excellent o2one and heat resistance. Additional protection is gained ia blends with a small amount of EPDM mbber (126). 

Tin—Nickel. AHoy deposits having 65% fin have been commercially plated siace about 1951 (135). The 65% fin alloy exhibits good resistance to chemical attack, staining, and atmospheric corrosion, especially when plated copper or bron2e undercoats are used. This alloy has a low coefficient of friction. Deposits are solderable, hard (650—710 HV ), act as etch resists, and find use ia pfinted circuit boards, watch parts, and as a substitute for chromium ia some apphcafions. The rose-pink color of 65% fin is attractive. In marine exposure, tin—nickel is about equal to nickel—chromium deposits, but has been found to be superior ia some iadustfial exposure sites. Chromium topcoats iacrease the protection further. Tia-nickel deposits are bfitde and difficult to strip from steel. Temperature of deposits should be kept below 300°C. 

Phenols Substituted phenols 4-Methyl-2, 6-di-t-butylphenol (VI) No Often used in non-toxic formulations. Very low level of staining. Widely used to protect polymers during synthesis and fabrication. Volatility restricts high-temperature and long-term use. 

Since this bloom is brittle, it is broken by flexing. Therefore, waxes only protect under static conditions. For serving conditions which involve continuous flexing, /j-phenylenediamines (A, A -alkyl-aryl derivatives) can be added. These chemical antiozonants scavenge the ozone before it reacts with the rubber. A barrier of ozonized products is created which protects both the rubber and antiozonant from further attack. However, p-phenylenediamines are staining compounds. Whenever colour is an important concern, blends of elastomers can be used elastomers loading should be higher than 30 phr to provide sufficient effectiveness. 

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