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Preparation of samples for single molecule fluorescence spectroscopy

Preparation of samples for single molecule fluorescence spectroscopy [Pg.159]

In this chapter we will briefly discuss the photophysical properties of commonly used dyes and, by reference to biomolecular systems studied by single molecule FRET in the literature, discuss the properties of commonly used dye pairs. We will then describe, in detail, the labelling and purification methods used in our laboratories to generate milligram quantities of a single chain protein labelled with two different dyes. Finally, we will discuss a variety of methods by which biomolecular systems have been tethered at or close to a surface, a necessary prerequisite for performing many of the experiments described in other chapters. [Pg.159]




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Fluorescence single-molecule

Fluorescence spectroscopy

Fluorescence spectroscopy sample preparation

Fluorescent preparation

Fluorescent spectroscopy

Molecule fluorescence

Molecule fluorescent

Molecule spectroscopy

Preparation of samples

Preparing samples for

Sample Fluorescence

Sample preparation for

Sampling molecules

Single fluorescent molecules

Single preparation

Single-molecule spectroscopy

Spectroscopy Sample preparation

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