Single-molecule fluorescence spectroscopy

Ha T, Ting A Y, Liang J, Caldwell W B, Deniz A A, Chemla D S, Schultz P G and Weiss S 1999 Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism Proc. Natl Acad. Sc/. USA 96 893-8... 

The importance of water molecules for the structural dynamics and the functioning of ribozymes was investigated by Rhodes and co-workers. They studied non-coded RNA using a combination of explicit solvent molecular dynamics and single molecule fluorescence spectroscopy approaches (Rhodes et al 2006). 

Finally, in Chapter 11 some advanced techniques are briefly described fluorescence up-conversion, fluorescence microscopy (confocal excitation, two-photon excitation, near-field optics, fluorescence lifetime imaging), fluorescence correlation spectroscopy, and single-molecule fluorescence spectroscopy. 

Smith, A. and Brockwell, D. 2006. Handbook of Single Molecule Fluorescence Spectroscopy. Oxford University Press, New York. 

Rothwell, P. J., Berger, S., Kensch, O., Felekyan, S., Antonik, M., Wohrl, B. M., Resde, T., Goody, R. S., and Seidel, C. A. (2003). Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptaseiprimer/template complexes. Proc. Natl. Acad. Sci. USA 100, 1655-1660. 

Weiss, S. 2000. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy. Nat. Struct. Biol. 7 724-729. 

Bowen, B.P., J. Enderlein, and N.W. Woodbury. 2003. Single-molecule fluorescence spectroscopy of TOTO on poly-AT and poly-GC DNA. Photochem.Photobiol. 78(6) 576-581. 

Ha, T., Ting, A. Y., Liang, J., Caldwell, W. B., Deniz, A. A., Chemla, D. S., Schultz, P. G., and Weiss, S (1999a). Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism. Proc. Natl. Acad. Sci. USA 96, 893-898. 

SECM instruments (77,78) will undoubtedly increase the scope and power of SECM. Further improvements in the power and scope of SECM has resulted from its coupling scanning probe or optical imaging techniques, such as AFM (57,79) or single-molecule fluorescence spectroscopy (80). The combined SECM-AFM technique offers simultaneous topographic and electrochemical imaging in connection to a probe containing a force sensor and an electrode component, respectively. 

Schuler, B., Lipman, E. A., Eaton, W. A., Probing the Free energy Surface for Protein Folding with Single molecule Fluorescence Spectroscopy, Nature 2002, 419, 743 747. 

C. Single-Molecule Fluorescence Spectroscopy with a Scanning Near-Field Optical Microscope... 

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