Isoenzymes lactate dehydrogenase

Gay, R. J. McComb, R. B. and Bowers, G. N. Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehyrogenase activity. Clin. Chem. (1968), U, 740-753. 

Rosalki, S. B. Standardization of isoenzyme assays with special reference to lactate dehydrogenase isoenzyme electrophoresis. Clin. Biochem. (1974), 2> 29-40. 

Bittar, P. G., Charnay, Y., Pellerin, L. etal. Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain. /. Cereb. Blood Flow Metab. 16 1079-1089,1996. 

Tang PT, Wang XP, Lu DS. 1992. Changes in lactate dehydrogenase isoenzymes of mitochondriaand microsomes in hepatic damage caused by phosphorus, sodium arsenite, and carbon tetrachloride. Zhongguo Yaolixue Yu Dulixue Zazhi 6(4) 292-296. 

Jaffe AS, Landt Y, Parvin CA,et al. Comparative sensitivity of cardiac troponin I and lactate dehydrogenase isoenzymes for diagnosing acute myocardial infarction. Clin Chem 42 1770-1776,1996. 

ST90 Jensen, A.E., Reikvam, A., Nordgird, S., Pedersen, K.G. and Asberg, A. (1990). Diagnostic accuracy of Kodak creatine kinase MB, Stratus creatine kinase MB, and lactate dehydrogenase isoenzyme 1 in serum after acute myocardial infarction. Clin. Chem. 36, 1847-1848. 

Methods for the Separation and Quantification of Lactate Dehydrogenase Isoenzymes... 

Chatterly S, Sun T, Lien Y. Diagnostic value of lactate dehydrogenase isoenzymes in cerebrospinal fluid. J Clin Lab Anal 1991 5 168-74. 

Moses GC, Ross ML, Henderson AR. Ten electrophoretic methods compared with a selected method for quantifying lactate dehydrogenase isoenzymes in serum. Clin Chem 1988 34 1885-90. 

Von Eyben FE, A systematic review of lactate dehydrogenase isoenzyme 1 and germ ceU tumors. Clin Biochem 2001 34 441-54. 

BIO. Brody, I. A., The significance of lactate dehydrogenase isoenzymes in abnormal human skeletal muscle. Neurology 14, 1091-1100 (1964). 

H16. Hughes, B. P., Lactate dehydrogenase isoenzymes and phospholipids in normal and diseased human muscle. In Muscle Diseases, Proc. Int. Congr., Milan, 1969 (J. N. Walton, N. Canal, and G. Scarlato, eds.), pp. 294-296. Excerpta Med. Found., Amsterdam, 1970. 

VlO. Vesell, E. S., pH dependence of lactate dehydrogenase isoenzyme inhibition by substrate. Nature 210, 421—422 (1966). 

Figure 16.6 lactate dehydrogenase isoenzymes were detected by their activity. Either lactate -F NAD + or pynivate -f NADH was a post-column addition. The reaction detector wasalOcmxS mm i.d. column packed with 150 pm glass beads (as diol derivative) at 40 °C. Detection principle NADH absorbs at 340 nm whereas NAD does not. 

D7. Denton, M. S., Bostick, W. D., Dinsmore, S. R., and Mrochek, J. E., Continuously referenced, on-line monitoring of creatine kinase and lactate dehydrogenase isoenzymes for use in clinical diagnostics. In "Biological/Biomedical Applications of Liquid Chromatography H (G. L. Hawk, ed.), pp. 165-191. Dekker, New York, 1979. 

K19. Kudirka, P. J., Schroeder, R. R., Hewitt, T. E., Torren, E. C., Jr., High-pressure liquid chromatographic separation of lactate dehydrogenase isoenzymes. Clin. Chem. 22, 471-474 (1976). 

M31. McKenzie, D., and Henderson, A. R., An artifact in lactate dehydrogenase isoenzyme patterns, assayed by fluorescence, occurring in the serum of patients with end-stage renal disease requiring maintenance hemodialysis. Clin. Ckim. Acta 70, 33-336 (1976). 

These data give the clinician an accurate picture of the nature of the diseased tissue. For a heart attack victim, only creatine phosphokinase isoenzyme 2 (CPK II, the predominant heart isoenzyme) will be elevated in the three days following the heart attack. CPK I (brain) and CPK III (skeletal muscle) levels will remain unchanged. Similarly, only LDH 1, the lactate dehydrogenase isoenzyme made in heart muscle, will be elevated. The levels of LDH 2-5 will remain within normal values. 

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