Sample preparation kit

In one study by Hood et al., 282 of 1153 identified proteins were identified by at least 2 unique tryptic peptides from FFPE prostate cancer (PCa) tissue.9 According to the gene ontology classification of the proteins identified, -65% of proteins were predicted to be intracellular proteins, while -50% of the total human proteome is predicted to be located in the intracellular compartment. Additionally, 20% of the proteins identified in the PCa tissue were classified as membrane proteins, which is significantly less than the predicted 40% for the human proteome. This relative disparity is not unexpected, considering the Liquid Tissue sample preparation kit lacks specific protocols for membrane protein extraction. The Liquid Tissue method has also been used for proteomics studies of a variety of FFPE tissue samples, including pancreatic tumors,28 squamous cell carcinoma,4 and oral human papillomavirus lesions.27... 

Sample Preparation Kit 12 Annex 3 Excerpt from Decision 71 ... 

The sample preparation kit (Picture 2) holds all equipment necessary to prepare the samples for GC/MS analysis on-site in four transport boxes (flight cases). It contains everything from the pH paper to the laboratory coat including the chemicals to conduct the sample preparation procedures. A reduced version of the sample preparation kit packaged in two pelli cases is available for inspections where this will suffice. 

This has particular limiting effects for sample preparation because of the detailed specifications given for equipment and chemicals approved for the sample preparation kit. The items of the sample preparation kit and not their purpose have been specified in detail. It turned out that some items, originally approved on the list, where not available on the market, except if custom produced for the OPCW. Because of these detailed specifications, developments in the area of sample preparations cannot easily be applied to on-site analysis. As a result, any change to sample preparation procedures that require an additional item of equipment, or more so an additional chemical, such as a different solvent (i.e. for sample clean up), cannot be implemented until the list of approved equipment is amended. 

The contents of the GC/MS sample preparation kit are based on the joint procedures provided by Finland and the United States of America. The application of these procedures resulted in the sample preparation kit consisting of the items, sufficient for the analysis of a maximum of four soils and a total... 

Items packed in the sample preparation kit are listed in Table 2 ... 

Table 2. Material and chemicals provided in the sample preparation kit...

There are restrictions in the selection of the sample preparation or introduction method when the laboratory is taken on site. Methods requiring supplies difficult to provide in field conditions, typically liquid nitrogen, cannot be applied on site. Thus, GC-based and ATR methods are not normally applicable on site. A suggestion for a basic on-site sample preparation kit is shown in Table 1. 

The sample preparation is minimal, and as many as 42 patient specimens can be tested in duplicate with one kit. 

A number of different testing kits based on immunoassay technology are available for rapid field determination of certain groups of compounds, such as benzene-toluene-ethylbenzene-xylene (EPA 4030) or polynuclear aromatic hydrocarbons (EPA 4035, Polycyclic Aromatic Hydrocarbons by Immunoassay). The immunoassay screening kits are self-contained portable field kits that include components for sample preparation, instrumentation to read assay results, and immunoassay reagents. 

Martinez, A. and Byrnes, A. The HP85070A dielectric probe kit tool sensitivity, sample preparation, and sampling procedures. 

The same test kit has been also applied to detect all members of the tetracycline group of antibiotics in kidney and meat tissue (72), although its crossreactivity varied from 4-5% for oxytetracycline and doxycycline to 100% for tetracycline and chlortetracycline. However, the applied sample preparation procedure... 

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