Tissue samples preparation

Chen XM, Dallas CE, Muralidhara S, et al. 1993. Analyses of volatile C2 haloethanes and haloethenes in tissues Sample preparation and extraction. J Chromatogr 612 199-208. 

TWO PHILOSOPHICALLY DIFFERENT APPROACHES FOR CELL/TISSUE SAMPLE PREPARATION... 

TABLE 11.1 Comparison between Two Philosophically Different Approaches for Cell/Tissue Sample Preparation... 

Several proteomic studies using archival FFPE tissues have been reported in recent years. Some involve the analysis of very small tissue samples prepared... 

In one study by Hood et al., 282 of 1153 identified proteins were identified by at least 2 unique tryptic peptides from FFPE prostate cancer (PCa) tissue.9 According to the gene ontology classification of the proteins identified, -65% of proteins were predicted to be intracellular proteins, while -50% of the total human proteome is predicted to be located in the intracellular compartment. Additionally, 20% of the proteins identified in the PCa tissue were classified as membrane proteins, which is significantly less than the predicted 40% for the human proteome. This relative disparity is not unexpected, considering the Liquid Tissue sample preparation kit lacks specific protocols for membrane protein extraction. The Liquid Tissue method has also been used for proteomics studies of a variety of FFPE tissue samples, including pancreatic tumors,28 squamous cell carcinoma,4 and oral human papillomavirus lesions.27... 

Plasma and Tissue Sample Preparation for Anthocyanin Analysis... 

Subramanian, K.S. Determination of metals in biofluids and tissue sample preparation methods for atomic spectroscopic technique. Spectrochim. Acta Part B 51, 291-319 (1996)... 

Comparison of immunocytochemical localization of the 1° antibody binding with known antibody or label for the correct protein or colocalization uses an immunocy-tochemical method of tissue sample preparation to determine whether the labeling is the same as the 1° antibody. 

Optical coherence tomography (OCT) is similar to ultrasound, except that light, rather than acoustic vibrations, are used as the signal source. Like ultrasound, an image can be composed from scans of light reflected from tissue interfacial boundaries. OCT has better resolution than MRI or ultrasound, and there is no ionizing radiation or special tissue sample preparation involved. 

Figure 4. Schematic of the tissue sample preparation protocol.

CD, JV, GY developed the cell culture and tissue sample preparation methods. SA, ST, and JM provided the Sd-FFF separation methods. All authors participated in the data analysis and manuscript preparation. 

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