Saccharomyces cerevisiae chromosomes

Saccharomyces cerevisiae Chromosome loss - - Mayer and Goin 1994... 

Markers. Methods for making HMW markers in the lab are described in Birren and Lai (1993). Beginners should purchase commercially available markers, including the 1-kb ladder (GIBCO-BRL 15615-016), Hindlll-digested X DNA (0.5-23 kb), low-mole-cular-weight (0.5-150 kb) marker (NEB 350), X concatamers (50-1000 kb) (Bio-Rad 170-3635), Saccharomyces cerevisiae chromosomes (Bio-Rad 170-3605), and Schizosaccharomyces pombe chromosomes (3-7 Mb). 

Hunt I thought you could see Saccharomyces cerevisiae meiotic chromosomes quite nicely. 

Weeks et al. 1979) or DNA damage (Nakamura et al. 1987) following hexachloroethane treatment. Similar results were reported for eukaryotic cells. Hexachloroethane did not cause gene mutation in cells harvested from the stationary growth phase (Bronzetti et al. 1989) or DNA damage in yeast (Saccharomyces cerevisiae) (Simmon and Kauhanen 1978), chromosomal aberrations in fungi (Aspergillus nidulans) (Crebelli et al. 

Wei Y, Yu L, Bowen J, Gorovsky MA, Allis CD (1999) Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1) 99—109 Wyatt HR, Liaw H, Green GR, Lustig AJ (2003) Multiple roles for Saccharomyces cerevisiae histone H2A in telomere position effect, Spt phenotypes and double-strand-break repair. Genetics 164(l) 47-64... 

The gene that encodes H2A.Z is essential in Drosophila [42], Tetrahymena [43], and mice [44]. Deletion of the gene that encodes H2A.Z in yeast results in slow growth and chromosome segregation defects in Schizosaccharomyces pompe [38], and in slow growth and defects in gene regulation in Saccharomyces cerevisiae [45-48]. 

Whittaker, S.G, Zimmermann, F.K., Dicus, B., Piegorsch, WW, Resnick, M.A. Fogel, S. (1990) Detection of induced mitotic chromosome loss in Saccharomyces cerevisiae—an interlaboratory assessment of 12 chemicals. Mutat. Res., 241, 225-242... 

Carbon tetrachloride was not mutagenic in bacteria. It induced intra-chromosomal and mitotic recombination but not aneuploidy in Saccharomyces cerevisiae, aneuploidy was detected in another single study in Aspergillus nidulans. In vivo, in a single study with Drosophila melanogaster, no sex-linked recessive mutations were observed. 

SCH, Saccharomyces cerevisiae RSI 12, intra-chromosomal + NT 4000 Schiestl etal. (1989)... 

Detection of induced mitotic chromosome loss in Saccharomyces cerevisiae—An interlaboratory assessment of 12 chemicals. Mutat. Res., 241, 225-242... 

Chlorodifluoromethane is mutagenic to Salmonella typhimurium but it did not induce either mutation or gene conversion in Saccharomyces cerevisiae. Chlorodifluoromethane did not induce mutations at the hprt locus or imscheduled DNA synthesis in mammalian cell lines in the presence or absence of an exogenous metabolic activation system. In vivo, it did not induce chromosomal aberrations in bone-marrow cells or dominant lethal effects (lARC, 1987b). These conclusions are supported by a more recent review (WHO, 1991). 

The genetically engineered Saccharomyces cerevisiae 424A (LNH-ST) was used for fermentation of lignocellulosic hydrolysates to ethanol. S. cerevisiae 424A (LNH-ST) was constructed by integrating multiple copies of XD, XR, and XK into the chromosomes of S. cerevisiae ATCC 4124 accord-... 

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