Saccharomyces cerevisiae genetic engineering

Friberg, A., Johanson, T., Franzen, J. etal. (2006) Efficient bioreduction of bicyclo[2.2.2]octane-2,5-dione and bicyclo[2.2.2]oct-7-ene-2,5-dione by genetically engineered Saccharomyces cerevisiae. Organic and Biomo-... 

Farhi, M., Dudareva, N., Masci, T. et al. (2006) Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae. Journal of Biotechnology, 122, 307-315. 

The genetically engineered Saccharomyces cerevisiae 424A (LNH-ST) was used for fermentation of lignocellulosic hydrolysates to ethanol. S. cerevisiae 424A (LNH-ST) was constructed by integrating multiple copies of XD, XR, and XK into the chromosomes of S. cerevisiae ATCC 4124 accord-... 

Hepatitis B (recombinant) Vaccine is a noninfectious rDNA hepatitis B vaccine containing purified surface antigen of the virus obtained by culturing genetically engineered Saccharomyces cerevisiae cells, which carry the surface antigen gene of the hepatitis B... 

Over the past 30 years, a few researchers have reported the presence of xylose isomerase in a number of yeasts and fungi capable of rapid xylose metabohsm. Because of difficulties in using genetically engineered Saccharomyces, Freer et al. [38] re-examined Rhodosporidium toruloides to see if they could confirm an earlier report that this yeast produces xylose isomerase. They reasoned that the heterologous expression of an eukaryotic enzyme could fadfitate genetic engineering of xylose metabolism in S. cerevisiae. Unfortunately, they found that R. toruloides uses an oxidoreductase system fike other eukaryotes. Other approaches, however, have been more successful. 

Numerous structural and mechanistic studies have been made with them.620 629 633-635 The gene for a lipase from Candida rugosa has been synthesized using codons that maximize its expression in Saccharomyces cerevisiae and which allow for further genetic engineering of the lipase.636... 

Helle, S., Cameron, D., et al., Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of Saccharomyces cerevisiae. Enzyme Microbial Technology 2003, 33 (6), 786-792. 

The polyphenolic adhesive protein of the mussel Mytilus edulis is an unusual protein composed mainly of repetitive decapeptide and hexapeptide sequences. In the mussel, the protein is first produced in a precursor form and is converted to an adhesive by post-translation-al modification. To develop an efficient renewable resource for production of the polyphenolic protein, we have used genetic engineering technology. cDNA sequences encoding portions of the polyphenolic protein were identified and expressed in the yeast Saccharomyces cerevisiae. 

Dejong, J.M., Liu, Y.L., Bollon, A.P. (2006) Genetic engineering of Taxol bios)mthesis in Saccharomyces cerevisiae. Biotechnol. Bioeng., 93, 212-24. 

Yeo ET., Kwon HB., Han SE., Lee JT., Ryu JC., Byu MO. Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae. Molecules and Cells 2000 10(3) 263-268. 

Genetic engineering of Saccharomyces cerevisiae yeast by transfomiation... 

Figure 9.19 There are two options to genetically engineer extraneous malate utilisation in order to deacidify wine. One approach utilises the Schizosaccharomyces pombe malate transporter gene (mael) and the O. oeni malolactic enzyme gene (mleA), enabling yeast to perform malolactic fermentation in parallel with alcoholic fermentation. Alternatively, Saccharomyces cerevisiae can be modified by the introduction of mael and the S. pombe malic enzyme gene (mae2), thereby enabling the conversion of malate into ethanol.

Volschenk, H., Viljoen-Bloom, M., Van Staden, J., Husnik, J., Van Vuuren, H. J. J. (2004). Genetic engineering of an industrial strain of Saccharomyces cerevisiae for L-mahc acid degradation via an efficient malo-ethanoUc pathway. South African Journal for Erwlogy and Viticulture, 25, 63-13. 

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