RT-PCR reverse

RT-PCR reverse transcriptase polymerase chain reaction RTV ritonavir... 

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

List of Abbreviations cDNA, complementary DNA ddH20, double-distilled H2O dNTP, deoxyribonu-cleotide triphosphate EDTA, ethylenediaminetetraacetic acid MgCl2, magnesium chloride mRNA, messenger ribonucleic acid NaOH, sodium hydroxide PCR, polymerase chain reaction qRT PCR, quantitative reverse transcriptase polymerase chain reaction RNase, ribonuclease RT PCR, reverse transcriptase polymerase chain reaction UTR, untranslated region... 

Abbreviation Bp, nucleotide base pairs cDNA, complementary DNA ChIP, chromatin Immunoprecipi-tation Cy5, cyanine 5-dCTP Cy3, cyanine 3-dCTP ESTs, expressed sequence tags FDR, false discovery rate MIAME, minimum information about a microarray experiment mRNA, RNA, messenger NIA, National Institutes of Aging RFUs, relative fluorescence units RT-PCR, reverse transcriptase polymerase chain reaction SAGE, serial analysis of gene expression SAM, significance analysis of microarrays... 

AGE — agarose gel electrophoresis, BAL = bronchoalveolar fluid, FAM = 6-carboxyfluorescein label, MGB = minor groove binder, n.s. - not specified, RAPD = randomly amplified polymorphic DNA, RT-PCR = reverse transcription PCR, TAMRA = 6-carboxytetramethylrhodamine label. 

TM, seven transmembrane domain DOR, Drosophila odorant receptor EAG, electroantennogram GFP, green fluorescent protein GPCR, G-protein coupled receptor GR, gustatory receptor nsyb, neuronal synaptobrevin OR, odorant receptor OSN, olfactory sensory neuron PCR, polymerase chain reaction PN, projection neuron RT-PCR, reverse transcription-polymerase chain reaction SOG, subesophageal ganglion. 

List of Abbreviations CD/MRV, Common Disease/Multiple Rare Variants DAAO, D-amino acid oxidase DAOA, D-aminoacid oxidase activator EST, expression sequence tag GEO, Gene Expression Omnibus MAF, minor allele frequency NIMH, National Institute of Mental Health NMDA, N-methyl D-aspartate RT-PCR, reverse transcription-PCR... 

To determine which protein has been tagged, RT-PCR (reverse transcription-polymerase chain reaction) can then be performed using a PCR primer from the GFP-coding sequence. A initial collection of over 100 tagged clones has been created (Jarvik et al. 2002) (http //cdtag.bio.cmu.edu). 

RT-PCR Reverse transcription-polymerase chain reaction TVC Transvenous cholangiography... 

Abbreviations used in text CYP, cytochrome P450 HMPA, hexamethylphosphoramide NDEA, N-nitrosodiethylamine NNK, 4-(methylnitrosamino)-l-(3-pyridyl)-l-butanone CPR, NADPH-cytochrome P450 reductase RT-PCR, reverse transcriptase-polymerase chain reaction TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin AFB1, aflatoxin Bl BaP benzo(a)pyrene G.I., gastrointestinal. 

PCR or RT-PCR) Reverse transcribe mRNA to generate cDNA Genotype specific probes... 

RT-PCR, Reverse transcription-polymerase chain reaction NASBA, nucleic acid sequence based amplification. For current FDA listings, see http //www.accessdata.fda.gov/scripts/cdrli/cfdocs/cERL/lLsting.cfiii. 

NA, not available HR, hormone receptor RT-PCR, reverse transcriptase polymerase chain reaction FFPE, formalin-fixed paraffin-embedded MCI, molecular grade index. 

RSCAAL Remote Sensing Chemical Agent Alarm RT-PCR reverse transcriptase polymerase chain reaction RVF Rift Valley fever virus... 

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