Amplification Nucleic acid sequence based

Lamhoujeb, S., Fliss, I., Ngazoa, S. E., and Jean, J. (2008). Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification. Appl. Environ. Microbiol. 74, 3349-3355. 

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

The quantitation of HCV RNA in serum may be important in predicting and monitoring response to antiviral therapy (Davis, 1994). A variety of methods have been used to quantitate HCV RNA, including endpoint dilution RT-PCR, competitive PCR, multicyclic RT-PCR, nucleic acid sequence-based amplification, RT-PCR with a single internal quantitation standard, and bDNA (Chazouilleres et al, 1994 Detmer et al., 1996 Ishiyama et al, 1992 Kaneko et al., 1992 Klevits et al., 1991 Kobayashi etal., 1993 Miskovsky etal., 1996). 

Compton, J. (1991). Nucleic acid sequence-based amplification. Nature 350,91-92. 

Amplification of nucleic acids also include RNA amplifications. For instance, the heat-shock mRNA (103-mer) in viable Cryptosporidium parvum in water treatment plants was detected after amplification by nucleic acid sequence-based amplification (NASBA). After amplification, a sandwich hybridization assay was subsequently conducted within a PDMS channel [955]. 

Gulliksen, A., Solli, L., Karlsen, F., Rogne, H., Hovig, E., Nordstrom, T., Sirevag, R., Real-time nucleic acid sequence-based amplification in nanoliter volumes, Anal. Chem. 2004, 76, 9-14. 

NASBA nucleic acid sequence-based amplification... 

Transcription-based amplification methods are modeled after the replication of retroviruses. These methods are known by various names including nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA)/ and self-sustained sequence repfi-cation (3SR) assays. Isothermal target amplification, using the collective activities of reverse transcriptase, RNase H, and RNA polymerase, is common to these methods. As illustrated in Figure 37-6, the method may be applied to... 

Figure 37-6 Schematic diagram of the nucleic acid sequence-based amplification (NASBA) method applied to single-stranded RNA and double-stranded (ds)DNA.The method is based on an extension of primer I (containing aT7 promoter) by reverse transcriptase (RT), degradation of the RNA strand by RNase H (or heat denaturation for double-stranded DNA), synthesis of the second strand of DNA by reverse transcriptase, and RNA synthesis byT7 RNA polymerase (pol). With RNA synthesis, the system enters the cyclic phase. Solid lines, DNA dashed lines, newly synthesized DNA wavy lines, RNA. (Reprinted by perm/ssion of the publisher from vanGemen B, Kievits T, Nara P, et al. Qualitative and quantitative detection of HIV-1 RNA by nucleic acid sequence-based amplification. AIDS, 7 Suppl 2 SI07-SI 10, 1993. 1993 Rapid Science Publishers.)...

RT-PCR, Reverse transcription-polymerase chain reaction NASBA, nucleic acid sequence based amplification. For current FDA listings, see http //www.accessdata.fda.gov/scripts/cdrli/cfdocs/cERL/lLsting.cfiii. 

Fox JD, Han S, Samuelson A, Zhang Y, Neale ML, Westmoreland D. Development and evaluation of nucleic acid sequence based amplification (NASBA) for diagnosis of enterovirus infections using the NuchSens Basic Kit. J Chn Virol 2002 24 117-30. 

Oldenberg N, Lam K, Khan M, Top B, Tacken N, McKie A, et al. Evaluation of human cytomegalovirus gene expression in thoracic organ transplant recipients using nucleic acid sequence-based amplification. Transplantation 2000 70 1209-15. 

Deiman, B., van Aarle, P, and Sillekens, P. (2002) Characteristics and applications of nucleic acid sequence-based amplification (NASBA). Mol Biotechnol. 20, 163-179... 

Real-time detection was also used for an isothermal amplification procedure termed nucleic acid sequence-based amplification, for the detection of artificial human papilloma virus sequences in SiHa cells. This method was not applied to real samples, but demonstrates the clinical applicability of additional microchip enzymatic amplification methods. Not all clinical microchip PCR amplifications looked for infectious agents, however. Cheng et al. showed a microchip PCR method for multiplex amplifications of dystrophin gene fragments for the detection of mutations associated with muscular dystrophy. 

Ch. 36. Nucleic Acid Sequence-Based Amplification (NASBA ),... 

In addition to PCR, there are many other technologies to amplify nucleic acids. For example, ligation-based amplification or ligase chain reaction uses sequence-directed oligonucleotide primers and thermostable DNA ligase to assay point mutations, deletions, or insertions in DNA. Strand-displacement amplification uses the inherent strand-displacement activity of DNA polymerases to conduct DNA amplification at a constant temperature. Transcription-based methods such as nucleic acid sequence-based amplification (NASBA) involve in vitro RNA transcription. NASBA and most other transcription-based... 

Lo W-Y, Baeumner AJ (2007) Evaluation of internal standards in a competitive nucleic acid sequence-based amplification assay. Anal Chem 79(4) 1386-1392... 

Baeumner AJ, Humiston MC, Montagna RA, Durst RA (2001) Detection of viable ooeysts of Cryptosporidium parvum following nucleic acid sequence based amplification. Anal Chem 73(6) 1176-1180... 

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