RT-PCR reverse transcription-polymerase

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

TM, seven transmembrane domain DOR, Drosophila odorant receptor EAG, electroantennogram GFP, green fluorescent protein GPCR, G-protein coupled receptor GR, gustatory receptor nsyb, neuronal synaptobrevin OR, odorant receptor OSN, olfactory sensory neuron PCR, polymerase chain reaction PN, projection neuron RT-PCR, reverse transcription-polymerase chain reaction SOG, subesophageal ganglion. 

To determine which protein has been tagged, RT-PCR (reverse transcription-polymerase chain reaction) can then be performed using a PCR primer from the GFP-coding sequence. A initial collection of over 100 tagged clones has been created (Jarvik et al. 2002) (http //cdtag.bio.cmu.edu). 

RT-PCR Reverse transcription-polymerase chain reaction TVC Transvenous cholangiography... 

RT-PCR, Reverse transcription-polymerase chain reaction NASBA, nucleic acid sequence based amplification. For current FDA listings, see http //www.accessdata.fda.gov/scripts/cdrli/cfdocs/cERL/lLsting.cfiii. 

Table 2. DMRTl expression in vertebrates. GSD-genetic sex determination, TDS-temperature dependence sex determination, T-testis/genital ridge in male embryo, O-ovary/ genital ridge in female embryo, K-kidney, L-liver, H-heart, M-muscle, LU-lung, S-spleen, ISH-in situ hybridisation, RT-PCR-reverse transcription-polymerase chain reaction, qRT-PCR-quantitative RT-PCR, NB-Northern blot, DB-Dot blot, IHC-immunohistochemistry, WB- Western blot, RPA-RNase protection assay.

RT-PCR, reverse transcription-polymerase chain reaction ASO, allele-specific oligonucleotide hybridization SSCP, single-stranded conformational polymorphism analysis. 

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