RT-PCR

Quantitative polymerase chain reaction, also called real-time RT-PCR or QPCR, is a method which employs insertion of a signal, such as fluorescence or enzyme activity, into PCR products generated by RT-PCR to determine the amount of messenger RNA (mRNA) in a tissue accurately. 

Castelnau C, Le Gal F, Ripault MP, Gordien E, Martinot-Peignoux M, Boyer N, Pham BN, Maylin S, Bedossa P, Deny P, MarcelUn P, Gault E (2006) Efficacy of peginterferon alpha-2b in chrome hepatitis delta relevance of quantitative RT-PCR for follow-up. Hepatology 44 728-735... 

RT-PCR A method used to quantitate mRNA levels that relies upon a first step of cDNA copying of mRNAs prior to PCR amplification and quantitation. 

In maize, many phenotypic mutants have been associated with cloned genes by a combination of HPLC analysis of specific intermediate metabolite accumulation, RT-PCR and immunolocalization of candidate genes, and recombinant inbred mapping of candidate cDNAs. Psyl was cloned by transposon tagging and later shown to be functional in the color complementation system and to be the specific... 

Northern blot analysis revealed that expression of NHE-1 is tissue-specific with highest transcript abundance in stomach and minimal levels in liver and skeletal muscle. Moreover, there is physiological evidence that certain segments of the nephron lack a basolateral Na /H" exchanger [76]. Using RT-PCR, Krapf and Solioz [77] observed that NHE-1 transcripts are not detectable in SI and S2 segments of superficial nephrons. 

Ngazoa, E. S., Fliss, I., and Jean, J. (2008). Quantitative study of persistence of human norovirus genome in water using TaqMan real-time RT-PCR. J. Appl. Microbiol. 104, 707-715. 

Nuanualsuwan, S. and Cliver, D. Q. (2002). Pretreatment to avoid positive RT-PCR results with inactivated viruses. /. Virol. Methods 104, 217-225. 

RT-PCR reverse transcriptase polymerase chain reaction RTV ritonavir... 

The primary goal in the treatment of CML is to eradicate the Ph-positive clones. Elimination of the Ph is termed cytogenetic complete remission. If treatment produces RT-PCR-negative disease, this is termed molecular complete remission and indicates a several-log reduction over cytogenetic complete remission. An early goal of therapy is to achieve hematologic complete remission or to normalize peripheral blood. 

Gleevec ) is a tyrosine kinase inhibitor used as first-line therapy in the majority of patients with CML. As a potent tyrosine kinase inhibitor, imatinib inhibits phosphorylation of various proteins involved in cell proliferation. Imatinib works by binding to the ATP-binding pocket of BCR-ABL.7 The drug induces complete hematologic responses in more than 95% of patients and complete cytogenetic responses in about 80% of patients in chronic phase.8 Most patients have traces of the disease when measured by RT-PCR and are not cured of their disease. 

IHC, IF, real-time PCR (mRNA), ELISA, RT-PCR, DNA microarray Real-time PCR (mRNA), IF, ELISA, RT-PCR Real-time PCR (mRNA), RT-PCR, Northern, ISH, IHC, IF, ELISA RT-PCR, IHC, ISH (mRNA)... 

Membrane bound form can IHC, IF, WB, real-time 173, 174 act as adhesion molecule PCR, RT-PCR Secreted by T cells and RT-PCR, ISH, ELISA 138, 175... 

CX3CR1 H PBMCs, synovium, SF CD3, CD4, CD4 (CD28-), CD8, CD14, MLS, FLS, DC, EC Interaction between ligand and receptor can mediate strong cell adhesion, and induce T-cell activation, cytotoxicity, and proliferation IHC, RT-PCR., FACS 176-178, 180... 

SF, synovium DNA microarray, RT-PCR, ELISA, ISH (mRNA) Higher levels compared with OA and traumatic joint injury. Increased expression compared with OA but not PA. 38, 159, 160, 190... 

CXCL11 SF, synovium ELISA, RT-PCR Higher levels compared with OA but not PA. 160, 162... 

XCL1 Synovium RT-PCR (mRNA), ISH (mRNA), IHC Higher levels compared with OA. Expression restricted to lymphocytic infiltrate in RA. Immunostaining shows strong staining in RA compared with OA and reactive arthritis but not PA. 175... 

CXCR1 Synovium DNA microarray, RT-PCR, WB Higher levels compared with OA. 159... 

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