Guanidium thiocyanate

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

Transfer the powder into a Potter and add 10 ml of guanidium thiocyanate. ... 

Chomczynski, P and Saachi, N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159. 

Fig. 7.2 Illustration of the spectroelectrochemical characterisation of a laboratory scale DSSC with a nanocrystalline Ti02 film sensitised with the RuN3 dye, measured under simulated AM 1.5G solar irradiation (100 mW cm ) electrolyte composition methoxyproprionitrile with 0.6 M propylmethylimidazolium iodide, 0.1 M Lil, 0.1 M tert butylpyridine, and 0.1 M iodine or guanidium thiocyanate 5 pm thick Ti02 film (9 nm particles) and scattering layer, a J-V curve, b IPCE spectrum (Courtesy of Patricia Jesus, Coimbra Chemistry Centre). In an optimised RuN3 DSSC the /sc reaches a value 20 mV and the maximum incident photon to current efficiency reaches 85 % [25]...

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