Transposon-tagging

In maize, many phenotypic mutants have been associated with cloned genes by a combination of HPLC analysis of specific intermediate metabolite accumulation, RT-PCR and immunolocalization of candidate genes, and recombinant inbred mapping of candidate cDNAs. Psyl was cloned by transposon tagging and later shown to be functional in the color complementation system and to be the specific... 

Brutnell, T.P., Transposon tagging in maize, Funct. Integr. Genomics 2, 4, 2002. Brutnell, T.P. and Conrad, L.J., Transposon tagging nsing Activator (Ac) in maize, Meth. Mol. Biol. 236, 157, 2003. 

SUZUKI, Y, UEMURA, S., SAITO, Y., MUROFUSHI, N., SCHMITZ, G., THERES, K., YAMAGUCHI, I., A novel transposon tagging element for obtaining gain-of-function mutants based on a self-stabilizing Ac derivative, Plant Mol. Biol., 2001, 45, 123-131. 

BISHOP, G.J., HARRISON, K., JONES, J.D., The tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family, Plant Cell, 1996, 8, 959-969. 

Martin, C. et al.. Molecular analysis of instability in flower pigmentation of Antirrhinum majus, following isolation of the pallida locus by transposon tagging. EMBO J., 4, 1625, 1985. 

McLaughlin, M., and Walbot, V., 1987, Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization, Genetics 117 771-776. 

Walbot, V., 1992, Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis, Annu. Rev. Plant Physiol. Plant Mol. Biol. 43 49-82. 

Other useful sites for yeast genome analysis include Saccharomyces cerevisiae Promoter Database, listing known regulatory elements and transcriptional factors in yeast TRansposon-Insertion Phenotypes, Localization, and Expression in Saccharomyces (TRIPLES) database, which tracks the expression of transposon-induced mutants and the cellular localization of transposon-tagged proteins, and the Saccharomyces Cell Cycle Expression Database, presenting the first results on changes in mRNA transcript levels dming the yeast cell cycle. 

Figure 2. A. Genomic mapping of transposon-tagged insertions conferring NP-1 -resistance to wild type Synechocystis. Arrows show the insertion sites, asterisks show the mutation sites determined by sequencing.

F. Kenneth Nelson, Hiroshi Iwasaki, Karl Hagers, Mark Gerstein, Perry Miller, G. Shirleen Roeder and Michael Snyder, Large-scale analysis of the yeast genome by transposon tagging and gene disruption, Nature, 402 (1999), 413 18. 

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