Real-time polymerase chain reaction RT-PCR

Real-time polymerase chain reaction (RT-PCR), microarray... 

In another example, real-time polymerase chain reaction (RT-PCR) was used to determine expression levels of the 48 known human ABC-type transporters in the NCI-60 cell lines, and these levels were correlated with sensitivity to 1429 candidate anticancer drugs (149, 150). Patterns of expression correlated moderately well with tissue of origin and were independent of sequence homology among... 

Reverse transcription-polymerase chain reaction (RT-PCR) has been widely used for the detection of cytokine gene expression in clinical samples (W18, K5). However, conventional RT-PCR only offers a semiquantitative analysis. Recently, the Perkin-Elmer Corporation (Wellesley, MA) developed the TaqMan cytokine gene expression plate for real-time, in vitro quantitative evaluation of a panel of human cytokine gene expression using fluorescence detection. 

Because the 5-HT4 receptors mediate physiological effects in the heart, gut, and CNS (132), splice variants of this receptor are thought to be involved in atrial arrhythmia, irritable bowel syndrome, and neurodegenerative diseases. Medhurst et al. (132) used TaqMan real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) to investigate the mRNA distribution of 5-HT4 receptor C-terminal splice variants in multiple human CNS and peripheral tissues. They... 

S. J. Wall and D. R. Edwards, Quantitative reverse transcription-polymerase chain reaction (RT-PCR) a comparison of primer-dropping, competitive, and real-time RT-PCRs. Anal Biochem 300(2) 269-273 (2002). 

Barletta, J. (2006) Applications of real time immuno polymerase chain reaction (rt PCR) for the rapid diagnoses of viral antigens and pathologic proteins. Molecular Aspects of Medicine, 21, 224 253. 

RT-PCR Real-time polymerase chain reaction T3 Triiodothyronine T4 Thyroxine... 

The stem cell is able to differentiate in a sinusoidal MF of 800pT with frequency of 50Hz. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR)... 

Quantitative polymerase chain reaction, also called real-time RT-PCR or QPCR, is a method which employs insertion of a signal, such as fluorescence or enzyme activity, into PCR products generated by RT-PCR to determine the amount of messenger RNA (mRNA) in a tissue accurately. 

Figure 11 TaqMan RT-PCR assay. Abbreviation RT-PCR, real-time reverse transcriptase-polymerase chain reaction.

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