Restriction fragment polymorphisms

The frequency of active ras oncogenes in human bladder cancer (F6) associated with schistosomiasis was examined. Of nine squamous cell carcinomas of the bladder, none scored as positive in the regular DNA transfection assay. The restriction fragment polymorphism assay at codon 12 of the H-ras gene confirmed the absence of an activating mutation at this site in all samples. Western blotting analyses of the ras p21 proteins suggested a point mutation with codon 61 in one sample only. Enhanced expression of the ras p21 protein was demonstrated in four samples. 

Hamet, P., Kong, D., Pravenec, M., Kunes, J., Kren, V., Klir, P., Sun, Y.L., Tremblay, J. (1992). Restriction fragment length polymorphism of hsp70 gene, localized in the RTl complex, is associated with hypertension in spontaneously hypertensive rats. Hypertension 19,611-614. 

DEMONSTRATION OF LINKAGE TO AN R F L P (restriction fragment iength polymorphism)... 

Restriction endonucleases facilitate diagnosis of genetic diseases by revealing restriction fragment length polymorphisms. 

The differences in DNA sequence cited above can result in variations of restriction sites and thus in the length of restriction fragments. An inherited difference in the pattern of restriction (eg, a DNA variation occurring in more than 1% of the general population) is known as a restriction fragment length polymorphism,... 

Mizutani, H., Ohbayashi, K., Umetsu, K. and Hiraoka, N. 1993. Restriction fragment length polymorphisms of medicinal plants and crude drugs. 11. Analysis of Glehnia littoralis of different geographical origin. Biol. Pharm Bull. 16 611-612. 

Degradation of contaminants may occur with bacteria that have been isolated from pristine environments without established exposure to the contaminants, and exhibit no dependence on substrate concentration. For example, organisms from a previously unexposed forest soil were able to degrade 2,4,6-trichlorophenol at concentrations up to 5000 ppm, and terminal restriction fragment length polymorphism analysis revealed that at concentrations up to 500 ppm, the bacterial community was unaltered (Sanchez et al. 2004). 

The first human DNA SRM developed by NIST was designed in the late 1980 s to standardize Restriction Fragment Length Polymorphism (RFLP) procedures which at that time were very new developments in the application of DNA to forensic analysis. 

Budowle B and Baechtel FS (1990) Modifications to improve the effectiveness of restriction fragment length polymorphism typing appMed theories. Electrophoresis 1 181-187. 

A. Darras.se. S. Priou, A. Kotoujansky, and Y. Beilheau, PCR and restriction-fragment-length-polymorphism of a pel gene to identify Erwinia ccirotovoro in relation to potato disi, Appl. Environ. Microbiol. 60 1443 (1994). 

Sato, T. Hu, J. P Ohki, K. Yamaura, M. Washio, J. Matsuyama, J. Takahashi, N. Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes. Oral Microbiol. Immunol. 2003,18, 323-326. 

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