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Restriction fragment length polymorphism, PCR

PCR-linked restriction fragment length polymorphism (PCR-RFLP) analysis... [Pg.67]

Sun, Y.L. and Lin, C.S. 2003. Establishment and application of a fluorescent polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP method for identifying porcine, caprine, and bovine meats. J. Agric. Food Chem. 51, 1771-1776. [Pg.234]

RFLP, restriction fragment length polymorphism PCR, polymerase chain reaction SSCP, single-strand conformation polymorphism. [Pg.297]

A. Darras.se. S. Priou, A. Kotoujansky, and Y. Beilheau, PCR and restriction-fragment-length-polymorphism of a pel gene to identify Erwinia ccirotovoro in relation to potato disi, Appl. Environ. Microbiol. 60 1443 (1994). [Pg.408]

Saperstein, D. and Nickerson, J.M. (1991) Restriction fragment length polymorphism analysis using PCR coupled to restriction digests. BioTechniques 10, 488-489. [Pg.87]

Another popular technique for endophyte identification is RFLP (restriction fragment length polymorphism).In this technique, nuclear or mitochondrial DNA is extracted from the endophyte and digested with restriction endonucleases. The digestion products are separated by gel electrophoresis, and a unique pattern emerges for the fungus, similar to the patterns seen in the RAPD-PCR technique. [Pg.513]

More recently, the forensic application of DNA testing has dramatically enhanced the ability to determine the source of a blood sample. Two procedures are in forensic use restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR). [Pg.676]

In conclusion, these results further open the door to the creation of numerous minisatellite probes to survey for VNTR loci. Such probes can be used not only for restriction fragment length polymorphism (RFLP) analyses of the many biological applications outlined in the introduction, but they can also be employed to screen cloned libraries with the ultimate goal of determining the sequences flanking VNTR loci. The latter information provides perhaps the most accurate and rapid means of utilizing the variation at such loci via the PCR analysis of alternate alleles. [Pg.294]


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Fragment length

Length restriction

PCR

Restriction fragment length

Restriction fragment polymorphisms

Restriction fragments

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