Restriction site

Thermal denatnration anneal mutagenic primers which also introduce a unique restriction. site... 

This polylinker contains restriction sites for BamHl, EeoRL, Pst, Sal, Smal, Sphl, and Xbal. Indicate the location of each restriction site in this sequence. (See Table 11.5 of restriction enzymes for their cleavage sites.)... 

A vector has a polylinker containing restriction sites in the following order Sael, Xhol, Bglll, Xbal, and Clal. 

The differences in DNA sequence cited above can result in variations of restriction sites and thus in the length of restriction fragments. An inherited difference in the pattern of restriction (eg, a DNA variation occurring in more than 1% of the general population) is known as a restriction fragment length polymorphism,... 

A North American origin for the silverswords clearly emerged from the application of DNA-sequence techniques to members of the alliance and representative Californian species (Baldwin et al., 1990, 1991). The conclusions from these studies, based on chloroplast restriction site analysis were subsequently corroborated by ITS nuclear ribosomal DNA studies. A comprehensive review of these studies can be found in Carlquist et al. (2003). 

Schilling, E. E., Panero, J. L. and Eliasson, U. A. 1994. Evidence from chloroplast DNA restriction site analysis on the relationships of Scalesia (Asteraceae Hehantheae). Amer. J. Bot. 81 248-254. 

The function of the B domain has been confirmed by subcloning and preliminary kinetic measurements. We subcloned the AB domain of E. coli II, residues 348-637, after inserting a restriction site at a position corresponding to residue 348. The purified protein restored mannitol phosphorylation activity when measured with the A domain assay in Fig. 4A, and the B domain assay in Fig. 4B [42]. The B domain... 

Saiki RK, Schare S, Faloona F Muelis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Science 1985 Dec 20, 23o(4732) i35o-i354-... 

C4. Chang, J.-G., Chiou, S.-S., Pemg, L.-I, Chen, T.-C., Liu, T.-C., Lee, L-S., Chen, P-H., and Tang, T. K., Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency by natural and amplification created restriction sites Five mutations account for most G6PD deficiency cases in Taiwan. Blood 80,1079-1082 (1992). 

Figure 12.10 Some genes and restriction sites on the E. coli plasma pBR322. Eco RI, Pst I, and Sal I are restriction endonucleases and the sites cleaved by these enzymes.

---