Restriction fragment length polymorphism, RFLP

The first human DNA SRM developed by NIST was designed in the late 1980 s to standardize Restriction Fragment Length Polymorphism (RFLP) procedures which at that time were very new developments in the application of DNA to forensic analysis. 

Restriction fragment length polymorphism (RFLP) Variation between individuals in DNA fragment sizes cut by specific restriction enzymes. RFLPs are usually caused by mutation at a cutting site. 

In the formation of the zygote, all mitochondria are contributed by the ovum. Therefore, mtDNA is transmitted by maternal inheritance in a vertical, nonmendelian fashion. Strictly maternal transmission of mtDNA has been documented in humans by studies of restriction fragment length polymorphisms (RFLPs) in DNA from platelets. As exemplified by the disorders outlined above,... 

Southern Blot Analysis of Restriction Fragment Length Polymorphisms (RFLPs) for Genetic Testing... 

Restriction fragment length polymorphism (RFLP) of variable number tandem repeats (VNTR) For each person, a pair of homologous chromosomes is shown. 

Mapping of eukaryotic chromosomes has involved additional methods which are discussed in Sections E and G,l. These incluse radiation hybrid mapping,104 use of meiotic recombination, identification of restriction fragment length polymorphisms (RFLPs described in Section E,7), and use of expressed sequence tags (ESTs), short DNA sequences deduced from mRNA molecules transcribed from the DNA.105 106... 

More recently, the forensic application of DNA testing has dramatically enhanced the ability to determine the source of a blood sample. Two procedures are in forensic use restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR). 

Whereas DNA polymorphisms should be recognizable by sequence differences, it is usually more convenient to detect these polymorphisms by differences in the size of DNA fragments obtained with restriction enzymes. Differences observed in this way are called restriction fragment length polymorphisms (RFLPs). 

Map of restriction fragment length polymorphisms (RFLPs) closely linked to the cystic fibrosis (CF) gene. The inverted triangle near the right-hand end indicates the location of the AF508 mutation characteristic of most persons with cystic fibrosis disease. (Source ... 

Cameron, M.L., Levy, P., Nutman, T., Vanamala, C.R., Narayanan, P.R. and Rajan, T.V. (1988) Use of restriction fragment length polymorphisms (RFLPs) to distinguish between nematodes of pathogenic significance. Parasitology 96, 381-390. 

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