Restriction fragment-linked polymorphism

Restriction enzymes, sequence-dependent cleavage of DNA by, 12 497—498 Restriction fragment linked polymorphism (RFLP), 12 500 procedure, 12 103-104 Restriction sites, as genetic markers, 12 500... 

PCR-linked restriction fragment length polymorphism (PCR-RFLP) analysis... 

Map of restriction fragment length polymorphisms (RFLPs) closely linked to the cystic fibrosis (CF) gene. The inverted triangle near the right-hand end indicates the location of the AF508 mutation characteristic of most persons with cystic fibrosis disease. (Source ... 

As pointed out in the introduction the cold acclimation capacity is a quantitative trait and work is just beginning to link such complex traits to molecular markers using restriction fragment length polymorphism (RFLP). Eventually, also, information about genes involved in environmental stress can be expected from these experiments. 

Paran, I., Kesseli, R., and Michelmore, R. W. (1991) Identification of restriction fragment length polymorphisms and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines. Genome 34, 1021-1027. 

Southern blotting can be used to follow the inheritance of selected genes. Mutations within restriction sites change the sizes of restriction fragments and hence the positions of bands in Southern-blot analyses. The existence of genetic diversity in a population is termed polymorphism. The detected mutation may itself cause disease or it may be closely linked to one that does. Genetic diseases such as sickle-cell anemia, cystic fibrosis, and Huntington chorea can be detected by RELP analyses. 

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