Rat aortic smooth muscle cells

Cathapermal S, LavigneMC, Leong-Son M,Alibadi T, Ramwell PW (1998) Stereoisomer-specific inhibition of superoxide anion-induced rat aortic smooth-muscle cell proliferation by 17beta-estradiol is estrogen receptor dependent. J Cardiovasc Pharmacol 31 499-505... 

Figure 6. Semi quantitative evaluation of spontaneous adsorption of collagen IV conjugated with a fluorescence marker Oregon Green 488 (A) to polyethylene foils iradiated with ions (energy 30 keV, dose from lO to lO ions/cm ), and its correlation with the number of rat aortic smooth muscle cells initially adhering to the polymer on day 1 after seeding (B). Collagen was diluted in phosphate-buffered saline to the concentration of 0.02 mg/ml (10 pg/cm ) and incubated with the foils for 24 h at room temperature. Mean+SEM from 4-9 experiments. Student t-test for unpaired data [43].

Figure 8. Concentration of markers of adhesion (A) and differentiation (B) in rat aortic smooth muscle cells in cultures on polyethylene (PE) modified by irradiation with ions (energy 30 keV, doses from lO to lO ions/cm ). Measured by enzymatic immunosorbent assay (ELISA) per mg of protein, absorbances expressed in % of the values obtained on pristine non-modified PE. Mean + SEM from 4 experiments. Student t-test for unpaired data, p<0.05 p<0.01 compared to the values on pristine PE.

Figure 21. Morphology of rat aortic smooth muscle cells on day 1 (A, B) and 3 (C, D) after seeding on pristine unmodified PE foils (A, C) or foils irradiated with 3xl0 ions C+ cm-2 (B) or 3xl0 " ions O cm (D) energy 150 keV. Stained with hematoxylin and eosin, microscope Opton Axioplan, Leica, Germany. Bar=100 pm.

Figure 23. Morphology of rat aortic smooth muscle cells 48 h after seeding on high-density (A, C, E) and low-density (B, D, F) polyethylene. A, B pristine polymers, C, D plasma-irradiated polymers, E, F polymers irradiated with plasma and subsequently grafted with glycine. Cell membrane stained with Texas Red C2-maleimide, the nuclei counterstained withHoechst 33342. Olympus IX 50 microscope, digital camera DP 70. Bar=200 pm.

Weber TJ, Ou X, Hart C, et al. 1991c. Modulation of protein phosphorylation in rat aortic smooth muscle cells by 2,3,7,8-tetrachlorodibnezo-p-dioxin (TCDD) in vivo [Abstract 1335], Toxicologist 11 340. 

Dubey, R. K., Jackson, E. K., and Luscher, T. F. 1995. Nitric oxide inhibits angiotensin II-induced migration of rat aortic smooth muscle cell. Role of cyclic-nucleotides and angiotensin 1 receptors. J Clin Invest 96 141-149. 

Geisterfer, A. A., Peach, M. J., and Owens, G. K. 1988. Angiotensin II induces hypertrophy, not hyperplasia, of cultured rat aortic smooth muscle cells. Circ Res 62 749-756. 

Molloy, C. J., Taylor, D. S., and Weber, H. 1993. Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in rat aortic smooth muscle cells. J Biol Chem 268 7338-7345. 

A growth-linked signaling pathway that is stimulated by angiotensin II in neonatal cardiomyocytes, rat aortic smooth muscle cells and cardiac fibroblasts is the... 

Du J, Sperling LS, Marrero MB, Phillips L, Delafontaine P. 1996. G-protein and tyrosine kinase receptor cross-talk in rat aortic smooth muscle cells thrombin- and angiotensin Il-induced tyrosine phosphorylation of insulin receptor substrate-1 and insulin-like growth factor 1 receptor. Biochem Biophys Res Commun 218 934-939. 

Oda, A., Taniguchi, T., and Yokoyama, M. 2001. Leptin stimulates rat aortic smooth muscle cell proliferation and migration. Kobe 1. Med. Sd. 47 141-150. 

WONG, H.R., MENENDEZ, I.Y., Sesquiterpene lactones inhibit inducible nitric oxide synthase gene expression in cultured rat aortic smooth muscle cells., Biochem. Biophys. Res. Comm., 1999,262,375-380. 

Osaka K, Tyurina YY, Dubey RK, et al. Amphotericin B as an intracellular antioxidant protection against 2, 2 -azobis(2, 4-di-methylvaleronitrile)-induced peroxidation of membrane phospholipids in rat aortic smooth muscle cells. Biochem Pharmacol... 

Medina J, Cordier A, Wolf A. Cyclosporine A-induced contraction of isolated rat aortic smooth muscle cells. Life Sci 1997 61 L-32. 

An estimate of the relative importance of Ca2+-ATPase and Na+-Ca2+ exchange has been made in cultured rat aortic smooth muscle cells by Furukawa et al. 1988). At [Ca2+]i below 1 pM, the contribution of the former was significantly greater than that of the latter. 

In cultured canine tracheal smooth muscle cells, phorbol 12-myristate 13-acetate (PMA) inhibited the histamine-induced IP3 formation (Murray et al., 1989), in cultured rat aortic smooth muscle cells phorbol esters inhibited agonist-induced PIP2 hydrolysis, and in cultured rabbit aortic smooth muscle the serotonin-stimulated PI breakdown was blocked after acute exposure to phorbol esters (Lee and Severson, 1994). 

FIGURE 5 Immunoprecipitation of vimentin by anti-protein kinase G (PKG) from rat aortic smooth muscle cells (SMCs). PKG was immunoprecipitated from low-passaged (passage 1) SMCs the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose. Vimentin was visualized using mouse antivimentin in a Western blot. The antibody also recognizes isotopes of rabbit anti-bovine PKG, as indicated by the staining of the immunoglobulin G heavy chain. [Data from MacMillan-Crow and Lincoln (1994) with permission of the American Chemical Society.]... 

Owens GK, Thompson MM (1986) Developmental changes in isoactin expression in rat aortic smooth muscle cells in vivo. Relationship between growth and cytodif-ferentiation. J Biol Chem 261 13373-13380... 

Kocher 0, Gabbiani G (1986) Expression of actin mRNAs in rat aortic smooth muscle cells during development, experimental intimal thickening, and culture. Differentiation 32 245-251... 

Majesky MW, Benditt EP, Schwartz SM (1988) Expression and developmental control of platelet-derived growth factor A-chain and B-chain/Sis genes in rat aortic smooth muscle cells. Proc Natl Acad Sci USA 85 1524-1528... 

Bochaton-Piallat M-L, Gabbiani F, Ropraz P, Gabbiani G (1993) Age influences the replicative activity and the differentiation features of cultured rat aortic smooth muscle cell populations and clones. Arterioscler Thromb 13 1449-1455... 

Blank RS, Owens GK (1990) Platelet-derived growth factor regulates actin isoform expression and growth state in cultured rat aortic smooth muscle cells. J Cell Physiol 142 635-642... 

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