Aortic smooth muscle cells

Long-lasting vasoconstriction is produced by the ETs in almost all arteries and veins and several studies have shown that ET-1 causes a reduction in renal blood flow and urinary sodium excretion. ET-1 has been reported to be a potent mitogen in fibroblasts and aortic smooth muscle cells and to cause contraction of rat stomach strips, rat colon and guinea pig ileum. In the central nervous system, ETs have been shown to modulate neurotransmitter release. 

Human aortic smooth muscle cells Flow chamber 0.5-2.5Nm-2(24h) [10]... 

Chandrasekar B, Bysani S, Mummidi S. CXCL16 signals via Gi, phosphatidylino-sitol 3-kinase, Akt, I kappa B kinase, and nuclear factor-kappa B and induces cell-cell adhesion and aortic smooth muscle cell proliferation. J Biol Chem 2004 279(5) 3188-3196. 

Cathapermal S, LavigneMC, Leong-Son M,Alibadi T, Ramwell PW (1998) Stereoisomer-specific inhibition of superoxide anion-induced rat aortic smooth-muscle cell proliferation by 17beta-estradiol is estrogen receptor dependent. J Cardiovasc Pharmacol 31 499-505... 

Marczin N, Jilling T, Papapetropoulos A, Go C, Catravas JD 1996 Cytoskeleton-dependent activation of the inducible nitric oxide synthase in cultured aortic smooth muscle cells. Br J Pharmacol 118 1085—1094... 

Ares, M.P., Pom-Ares, MI, Moses, S, Thyberg, J, Jrmtti-Berggren, L, Beiggien, P, Hultgardh-Nilsson, A, Kallin, B, Nilsson, J., 2000, Tbeta-hydroxycholesterol induces Ca(2-l-) oscillations, MAPK activation and apoptosis in human aortic smooth muscle cells, Atherosclerosis 153 2 3-35. 

Brigehus-Flohe,R., Maurer, S., Lotzer, K., Bol, G., Kalhonpaa, H., Lehtolainen, P., Viita, H., and Yla-Herttuala, S., 2000, Overexpression ofPHGPx inhibits hydroperoxide-induced oxidation, NFkappaB activation and apoptosis and affects oxLDL-mediated proliferation of rabbit aortic smooth muscle cells, Atherosclerosis 152 307-316. 

Hughes, H., Mathews, B., Lenz, M.L., and Guyton, J.R., 1994, Cytotoxicity of oxidized LDL to porcine aortic smooth muscle cells is associated with the oxysterols 7-ketocholesterol and 7-hydroxycholesterol, Arteriosc/er. Thromb. 14 1177- 1185. 

S IP-dependent activation of the non-receptor tyrosine kinase Pyk2 was dissociated from p42/p44 MAPK in aortic smooth muscle cells (Guo et al,... 

Kozawa, O., Tanabe, K., Ito, H., Matsuno, H., Niwa, M., Kato, K. and Uematsu, T., 1999b, Sphingosine 1-phosphate regulates heat shock protein 27 induction by ap38 MAP kinase-dependent mechanism in aortic smooth muscle cells, Exp. Cell Res. 250 376-380. 

Kozawa, O., Yamamoto, T., tanabe, K., Akamatsu, S., Dohi, S. and Uematsu, T., 2000, Enhancement by sphingosine 1 -phosphate in vasopressin-induced phosphoinositide hydrolysis in aortic smooth muscle cell involvement ofp38 MAP kinase, J. Cell. Biochem. 18 46-52. 

Figure 6. Semi quantitative evaluation of spontaneous adsorption of collagen IV conjugated with a fluorescence marker Oregon Green 488 (A) to polyethylene foils iradiated with ions (energy 30 keV, dose from lO to lO ions/cm ), and its correlation with the number of rat aortic smooth muscle cells initially adhering to the polymer on day 1 after seeding (B). Collagen was diluted in phosphate-buffered saline to the concentration of 0.02 mg/ml (10 pg/cm ) and incubated with the foils for 24 h at room temperature. Mean+SEM from 4-9 experiments. Student t-test for unpaired data [43].

Figure 8. Concentration of markers of adhesion (A) and differentiation (B) in rat aortic smooth muscle cells in cultures on polyethylene (PE) modified by irradiation with ions (energy 30 keV, doses from lO to lO ions/cm ). Measured by enzymatic immunosorbent assay (ELISA) per mg of protein, absorbances expressed in % of the values obtained on pristine non-modified PE. Mean + SEM from 4 experiments. Student t-test for unpaired data, p<0.05 p<0.01 compared to the values on pristine PE.

Figure 21. Morphology of rat aortic smooth muscle cells on day 1 (A, B) and 3 (C, D) after seeding on pristine unmodified PE foils (A, C) or foils irradiated with 3xl0 ions C+ cm-2 (B) or 3xl0 " ions O cm (D) energy 150 keV. Stained with hematoxylin and eosin, microscope Opton Axioplan, Leica, Germany. Bar=100 pm.

Figure 23. Morphology of rat aortic smooth muscle cells 48 h after seeding on high-density (A, C, E) and low-density (B, D, F) polyethylene. A, B pristine polymers, C, D plasma-irradiated polymers, E, F polymers irradiated with plasma and subsequently grafted with glycine. Cell membrane stained with Texas Red C2-maleimide, the nuclei counterstained withHoechst 33342. Olympus IX 50 microscope, digital camera DP 70. Bar=200 pm.

Dubey RK, Gillespie DG, Imthurn B et al. Phytoestrogens inhibit growth and MAP kinase activity in human aortic smooth muscle cells. Hypertension 33, 177-182, 1999. 

In contrast to these results, 25-hydroxycholesterol (and also, 20-hy-droxycholesterol, 7-ketocholesterol, and diosgenin) in aortic, smooth-muscle cells effectively blocks the incorporation of acetate into lipid-linked oligosaccharides (and, also, into cholesterol7). Thus, less of the lipid-linked oligosaccharides were available for glycosylation of proteins. In harmony with the presumed, inhibitory mechanism was the observation that incorporation of mevalonate into lipid-linked oligosaccharides was not inhibited, and that mevalonate itself (the product formed by HMG-CoA reductase from HMG-CoA and NADPH) could reverse the inhibition of glycosylation of protein (see Scheme 1). 

Juan SH, Cheng TH, Lin HC, Chu YL, Lee WS. 2005. Mechanism of concentration-dependent induction of heme oxygenase-1 by resveratrol in human aortic smooth muscle cells. Biochem Pharmacol 69 41 48. 

Carreras, I., Rich, C. B., Panchenko, M. P., and Foster, J. A. (2002). Basic fibroblast growth factor decreases elastin gene transcription in aortic smooth muscle cells. / Cell. Biochem. 85, 592—600. 

Liu, J. M., and Davidson, J. M. (1988). The elastogenic effect of recombinant transforming growth factor-beta on porcine aortic smooth muscle cells. Biochem. Biophys. Res. Comm. 154, 895-901. 

Ooyama, T., Fukuda, K., Oda, H., Nakamura, H., and Hikita, Y. (1987). Substratum-bound elastin peptide inhibits aortic smooth muscle cell migration in vitro. Arteriosclerosis 7, 593-598. 

Weber TJ, Ou X, Hart C, et al. 1991c. Modulation of protein phosphorylation in rat aortic smooth muscle cells by 2,3,7,8-tetrachlorodibnezo-p-dioxin (TCDD) in vivo [Abstract 1335], Toxicologist 11 340. 

Chatteijee, S. (1991). Lactosylceramide stimulates aortic smooth muscle cell proliferation. Biockem. Biophys. Res. Commun. 181, 554-561. 

Dubey, R. K., Jackson, E. K., and Luscher, T. F. 1995. Nitric oxide inhibits angiotensin II-induced migration of rat aortic smooth muscle cell. Role of cyclic-nucleotides and angiotensin 1 receptors. J Clin Invest 96 141-149. 

Folh, F., Kahn, C. R., Hansen, H., et al. 1997. Angiotensin II inhibits insulin signaling in aortic smooth muscle cells at multiple levels. A potential role for serine phosphorylation in in-sulin/angiotensin II crosstalk. J Clin Invest 100 2158-2169. 

Geisterfer, A. A., Peach, M. J., and Owens, G. K. 1988. Angiotensin II induces hypertrophy, not hyperplasia, of cultured rat aortic smooth muscle cells. Circ Res 62 749-756. 

Leduc, I., and Meloche, S. 1995. Angiotensin II stimulates tyrosine phosphorylation of the focal adhesion-associated protein paxillin in aortic smooth muscle cells. J Biol Chem 270 4401 1404. 

Leduc, I., Haddad, P., Giasson, E., et al. 1995. Involvement of a tyrosine kinase pathway in the growth-promoting effects of angiotensin II on aortic smooth muscle cells. Mol Pharmacol 48 582-592. 

Molloy, C. J., Taylor, D. S., and Weber, H. 1993. Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in rat aortic smooth muscle cells. J Biol Chem 268 7338-7345. 

A growth-linked signaling pathway that is stimulated by angiotensin II in neonatal cardiomyocytes, rat aortic smooth muscle cells and cardiac fibroblasts is the... 

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