Quantitative real-time polymerase chain reaction

FIGURE3.10 UCP1 mRNA expression levels in epididymal WAT (Okada et al., 2011). Expression of UCP1 mRNA was estimated by quantitative real-time polymerase chain reaction. Relative values were presented as the ratio of UCP1 mRNA to GAPDH mRNA. 

Assimilable organic carbon (AOC) was determined in water using flow-cytometric enumeration and a natural microbial consortium as inoculum.134 Two bacterial species were used for the measurement of AOC in water, based on their respective 16S rDNA sequences. The AOC content in 41 water samples was determined with these two sets by quantitative real-time polymerase chain reaction (qRT-PCR).135... 

Lekanne Deprez, R.H. Fijnvandraat, A.C. Ruijter, J.M. Moorman, A.F.M. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green... 

Key Words Arthropod-borne disease quantitative real-time polymerase chain reaction vector-borne transmission vector competence. 

Demmer RT, Molitor JA, Jacobs Jr. DR, Michalowicz BS. Periodontal disease, tooth loss and incident rheumatoid arthritis results from the First National Health and Nutrition Examination Survey and its epidemiological follow-up study. J Clin Periodontal. 2011 38(11) 998—1006. Alvarez-Lafuente R, Fernandez-Gutierrez B. Potential relationship between herpes viruses and rheumatoid arthritis analysis with quantitative real time polymerase chain reaction. Ann Rheum Dis. 2005 64(9) 1357—1359. 

Kuboniwa, M. Amano, A. Kimura, K. R. Sekine, S. Kato, S. Yamamoto, Y. Okahashi, N. Iida, T. Shizukuishi, S. Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes. Oral Microbiol. Immunol. 2004,19,168-176. 

Fig. 9.4 Establishment of the quantitative methylation-specific polymerase chain reaction (MSP) analytical procedure. The ABI PRISM 7900 HT Sequence Detector system was used to perform real-time polymerase chain reaction (PCR) using MSP primers and bisulfite-modified template DNA. Upper panels Setting up the conditions to obtain the standard curves with 50% (A) or 25% (B) sequential dilution of the template. Lower panels The amplification curves on the left represent P-actin, unmethylated, and methylated MSP products, respectively, for reelin (RELN) (C). Amplification curves were compared at the set threshold before 40 cycles. Amplification curves from various samples are shown in the lower panel right (D)...

Hernandez M, Esteve T, Pla M (2005). Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J. Agric. Food Chem., 53 (18) 7003-7009. 

Gl. Gerard, C. J., Olsson, K., Ramanathan, R., Reading, C., and Hanania, E. G., Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards. Cancer Res. 58, 3957-3964 (1998). 

Femdndez, M., Del Rio, B., Linares, D. M., Martin, M. C., Alvarez, M. A. (2006). Real-time polymerase chain reaction for quantitative detection of histamine-producing bacteria use in cheese production. Journal of Dairy Science, 89, 3763-3769. http //dx.doi.org/10.3168/jds.S0022-0302(06)72417-l. 

Fite, A., Macfarlane, G.T. and Cummings, J.H. (2004) Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. Gut, 53, 523-529. 

Because the 5-HT4 receptors mediate physiological effects in the heart, gut, and CNS (132), splice variants of this receptor are thought to be involved in atrial arrhythmia, irritable bowel syndrome, and neurodegenerative diseases. Medhurst et al. (132) used TaqMan real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) to investigate the mRNA distribution of 5-HT4 receptor C-terminal splice variants in multiple human CNS and peripheral tissues. They... 

S. J. Wall and D. R. Edwards, Quantitative reverse transcription-polymerase chain reaction (RT-PCR) a comparison of primer-dropping, competitive, and real-time RT-PCRs. Anal Biochem 300(2) 269-273 (2002). 

Ueno, H., Yoshida, K., Hirai, T., Kono, F., Kambe, M., and Toge, T., Quantitative detection of carcinoembryonic antigen messenger RNA in the peritoneal cavity of gastric cancer patients by real-time quantitative reverse transcription polymerase chain reaction. Anticancer Res. 23, 1701-1708 (2003). 

The stem cell is able to differentiate in a sinusoidal MF of 800pT with frequency of 50Hz. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR)... 

Quantitative polymerase chain reaction, also called real-time RT-PCR or QPCR, is a method which employs insertion of a signal, such as fluorescence or enzyme activity, into PCR products generated by RT-PCR to determine the amount of messenger RNA (mRNA) in a tissue accurately. 

Nakamura, T., Sakaeda, T., Ohmoto, N., Tamura, T., Aoyama, N. et al., Real-time quantitative polymerase chain reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA levels in Caco-2 cell lines, human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarcinomas, Drug Metab. Dispos. 2002, 30, 4—6. 

Abrahamsen HN, Steiniche T, Nexo E, et al. Towards quantitative mRNA analysis in paraffin-embedded tissues using real-time reverse transcriptase-polymerase chain reaction. A methodological study on lymph nodes from melanoma patients. J. Mol. Diagn. 2003 5 34-41. 

Bustin SA, Nolan T. 2004. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech 15 155-166. 

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