Quantitative analysis of organic acids after separation by column chromatography

2 Quantitative analysis of organic acids after separation by column chromatography 

Organic acids are absorbed from an acidified aqueous solution by being passed through a chromatographic column of silica gel. After elution with a chloroform/butanol mixture, titration is carried out in the eluate itself with methanolic sodium hydroxide solution. This method is only recommended for dissolved organic acids after preliminary filtration of the water sample, where necessary. 

Dissolved organic acids in concentrations of 0.5 mmol/1 and above can be 

Glass filter crucible for 20 g silica gel 1000 ml shaking funnel 

Silica gel for chromatography, 50 - 200 mesh. To separate off any excessively fine material, elutriate the preparation with distilled water, let it 

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