Quantitation of Lipid Classes by Thin-Layer Chromatography with Flame Ionization Detection

Thin-layer chromatography (TLC) on silica gel is well known for its separation power for lipids and related compounds. The flame ionization detector (FID) is a universal analytical instrument that offers high sensitivity and linearity for carbon-containing organic compounds. The combination of TLC and FID led to the wide use of the Iatroscan TLC-FID for the analysis of lipid classes. The adoption of the Iatroscan TLC-FID in both academia and industry has generated sufficient data to indicate that TLC-FID is currently one of the most efficient tools for the quantitation of lipids classes (Ackman et al., 1990 Hammond, 1993). [Pg.491]

CAUTION Due to the hazardous nature of chloroform, procedures using chloroform must be conducted in a chemical fume hood. See appendix2b for more information. [Pg.491]

NOTE All reagents must be at least analytical grade or higher purity. [Pg.491]

Quantitation of Lipid Classes by Thin-Layer Chromatography with Flame Ionization Detection [Pg.492]

97 3 1 (v/v/v) hexane/diethyl ether/formic acid, fresh Lipid sample Methylene chloride [Pg.492]

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