High performance thin layer chromatography quantitative analysis

Sethi, ED. HPTLC, High Performance Thin-Layer Chromatography, Quantitative Analysis of Pharmaceutical Formulations, CBS Publisher Distributors, New Dehli (India) 1996, ISBN... 

Benzimidazole carbamates have generally been determined by HPLC (20,124-126), although other methods have been reported based on enzyme immunoassay (147) or in gas chromatography (124). The commercial availability of high-performance thin-layer chromatography (HPTLC) plates with layers of very different polarities has improved the sensitivity, selectivity, and analytical precision of TLC, which has led to a reconsideration of the use of this technique for qualitative and quantitative analysis (54). 

Liang, Y. Baker, M.E. Todd Yeager, B. Bonner Denton, M. Quantitative analysis of aflatoxins by high-performance thin-layer chromatography utilizing a scientifically operated charge-coupled device detector. Anal. Chem. 1996, 68, 3885. 

Mirza, M.A., Talegaonkar, S., and Iqbal, Z. 2012. Quantitative analysis of itraconazole in bulk, marketed, and nano formulation by validated, stability indicating high performance thin layer chromatography, J. Liq. Chromatogr. Relat. TechnoL, 35 1459-1480. 

TLC is a sensitive technique, and it is important to think small with respect to sample size. The highest resolution will always be obtained with the smallest sample commensurate with the ability to visualize the analyte. This is also one of the prerequisites for reproducibility, especially if densitometry is to be used for quantitation (see Chap. 13.8). Zones that are too concentrated re.sult in integrated curve areas that behave in a nonlinear way on regression plots. Depending on the nature of the analyte, sample. sizes as small as picogram amounts are not uncommon, especially in high-performance thin layer chromatography (HPTLC). This is especially true in the analysis of fluorescent substances. Thus, for ultimate sensitivity it is sometimes desirable that a fluorescent derivative of the analyte be prepared. 

Fell, R, D. (1990). The qualitative and quantitative analysis of insect hemolymph sugars by high performance thin-layer chromatography. Comp. Biochem. Physiol. 95A 539-544. 

In the last years new developments in the field of plate chromatography have led to high performance thin layer chromatographic (HPTLC) systems. HPTLC offer simultaneous and cost-efficient screening of anthocyanin samples and provides the possibility for quantitative analysis. 

Several technology leaps have taken place in separation sciences during the lifetime of the pharmaceutical industry. The development of chromatography at the end of the nineteenth century was the first of these revolutions and its transformation into thin-layer chromatography (TLC) provided the mainstay for quantitative analysis well into the second half of the twentieth century. With the development of gas chromatography (GC) after World War II and high-performance liquid chromatography (HPLC) two decades later, the age of fully instrumented separation science had arrived. 

In earlier times, thin-layer chromatography (TLC), polyamide chromatography, and paper electrophoresis were the major separation techniques for phenolics. Of these methods, TLC is still the workhorse of flavonoid analysis. It is used as a rapid, simple, and versatile method for following polyphenolics in plant extracts and in fractionation work. However, the majority of published work now refers to qualitative and quantitative applications of high-performance liquid chromatography (HPLC) for analysis. Llavonoids can be separated. 

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