Qualitative analysis liquid chromatography-mass spectrometry

Mohn T, Cutting B, Ernst B, Hamburger M (2007) Extraction and analysis of intact glucosinolates-a validated pressurized liquid extraction/liquid chromatography-mass spectrometry protocol for Isatis tinctoria, and qualitative analysis of other cruciferous plants. J Chromatogr 1166 142-151... 

Li C, Hong Y, Tan YX et al. Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry. Mo/ Cell Proteomics 2004,3399-409. 

Lant, M. S. Martin, L. E. Oxford, J. 1985. Qualitative and quantitative analysis of ranitidine and its metabolites by high-performance liquid chromatography-mass spectrometry. /. Chromatogr., 323,143-152. 

Often there is a need for structural identification of unknowns without available reference compounds and the identification can be done in connection with the chromatographic separation. One approach is to run measurements directly on-line using HPLC as the separation technique with UV-detection and monitoring at several wavelengths, but this is often not enough for safety identification. The last 15 years have seen a rapid development of combined liquid chromatography-mass spectrometry instrumentation, and this technique is the most valuable tool in qualitative analysis today (se below). In the absence of a reference compound some unknown substances e.g., isomers of the desired compound may require NMR for their definitive identification. 

Tracqui, A. Kintz, R Mangin, R Lugnier, A.A. Chaumont, A.J. A new rapid HPLC assay for the simultaneous determination of two histamine H2-receptor antagonists, cimetidine and ranitidine, in human plasma. J.Toxicol.CUn.Exp., 1988, 8, 387-394 Lant, M.S. Martin, L.E. Oxford, J. Qualitative and quantitative analysis of ranitidine and its metabolites by high-performance liquid chromatography-mass spectrometry. J.Chromatogr., 1985, 323, 143-152 [UV detection LC-MS]... 

Milk, milk proteins, and allergenic milk proteins are very well characterized. The tests that can be used for the determination of allergenic milk proteins range from well-documented protocols to newly developed methods. However, the choice of qualitative rapid tests is still scant and there is an increasing demand for suitable reference materials. A main goal for the future is the development of alternative reference methods such as liquid chromatography-mass spectrometry analysis without the use of antibodies. 

Wu, H., J. Guo, S. Chen et al. 2013. Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography-mass spectrometry./. Pharm. Biomed. Anal. 72 267-291. 

Dear, G.J., Fraser, I.J., Patel, D.K., Long, J., and Pleasance, S., Use of liquid chromatography-tandem mass spectrometry for the quantitative and qualitative analysis of an antipsychotic agent and its metabolites in human plasma and urine, /. Chromatogr. A, 794, 27, 1998. 

Simoes SS, Ajenjo AC, Franco JM, Vieira DN, Dias MJ (2009) Liquid chromatography/tan-dem mass spectrometry for the qualitative and quantitative analysis of illicit drugs and medicines in preserved oral fluid. Rapid Commun Mass Spectrom 23(10) 1451-1460. doi 10.1002/ rcm.4020... 

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