Milk allergens

An alternative mechanism is based on non-complement dependent cytotoxicity, e.g., food allergen stimulation of mechanisms of antibody dependent cellular cytotoxicity (ADCC), cytotoxicity of natural killer cells or macrophages. The contribution of ADCC in gastroenteropathy of children induced by cow s milk allergens was demonstrated by Owen et al. (1993). 

To complete this short review about the interest of using CPLLs in allergen discovery, a note is to be made for other published results of fungal allergen discovered in blood subsequent to invasive aspergillosis (90), bovine milk allergens (91), and hen s egg allergens (92). 

Top-down proteomics Cow s milk allergens Mixed fruit-juice (68)... 

Cocco, R.R., Jarvinen, K.M., Sampson, H.A., and Beyer, K. 2003. Mutational analysis of major, sequential IgE-binding epitopes in alpha sl-casein, a major cow s milk allergen. J Allergy Clin Immunol 112(2) 433—437. 

CHARACTERIZATION OF ALLERGENS IN COW S MILK STRUCTURE AND FUNCTION OF MILK ALLERGENS... 

Cow s milk contains about 30-35 g proteins per liter, of which caseins make 80% and whey proteins 20%. One of the best list of known allergens of animal origin, with a good description of milk allergen, is InformAll (http //www.foodallergens.ifr.html). 

Ehn, B.M., Ekstrand, B., Bengtsson, U., and Ahlstedt, S. 2004. Modification of IgE binding during heat processing of the cow s milk allergen beta-lactoglobulin. JAgric Food Chem 52(5) 1398-1403. 

Monaci, L., Tregoat, V., Van Hengel, A.J., and Anklam, E. 2006. Milk allergens, their characteristic and their detection in food. A review. Eur Food Res Technol 223 149-179. 

Human a-la is highly homologous to bovine a-la, with 66% identity (Aalberse and Stapel 2001). Human milk is free from P-lg, the main cow milk allergen, but in... 

CHANGES IN IMMUNOREACTIVITY AND ALLERGENICITY OF MILK ALLERGENS DURING TECHNOLOGICAL PROCESSES... 

Reduction of milk allergenicity is possible by a cross-linked reaction of milk proteins with microbial transglutaminase (m-TG) (Wroblewska et al. 2008). Diary products thus obtained possessed better functional properties with weaker syneresis and stronger curd. Also, the organoleptic properties of yoghurt prepared with m-TG make a favorable impression on potential allergic consumers. 

Several authors report coexisting clinical soy allergies in 5%-50% of patients with cows milk allergies (NDA Opinion 2004). It is unclear whether soy allergy represents a de novo sensitization or a cross-reaction of a soy protein component with caseins from milk (Rozenfeld et al. 2002). However, due to homology in the amino acid sequences of soybean and cow s milk allergens of 50%-70%, cross-reactivity is likely (Wilson et al. 2005). 

Alachua, Florida, www.ehsa-tek.com Neogen Corporation, Lansing, sandwich-type EIA utilizing biotin-avidin enhancement Veratox for Milk Allergen, Milk 2.5-25 mg/kg NA... 

Michigan, www.neogen.com sandwich-ELISA Reaveal for Total Milk Allergen, Milk NA 5 mg/kg... 

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