Protein enrichment with essential amino acid

Maize with enhanced protein content and enriched in essential amino acids is an important object of research and development to serve as a food basis for coimtries in Afiica, South and Middle America and Asia. 

A distribution of amino acids in dietary proteins can be obtained accordingly by taking both animal and plant proteins at a ratio of 1 3-4. Although plant proteins are lower cost, they are markedly deficient in some essential amino acids. Their protein efficiency is low without addition of deficient ammo acids. Enrichment of human and animal diets with free amino acids, such as lysine, methionine, threonine, and tryptophan, as a substitute for animal proteins, has proved successful. 

Modification via covalent enrichment of proteins/peptides with the limiting essential amino acids... 

So far, two processes involving membrane separation for canola protein isolation are considered promising, and both are currently under commercial development Researchers at the University of Toronto developed a membrane-based process for canola protein isolation from defatted meal [29,42], in which, after precipitation, the soluble proteins were ultraflltered to be concentrated and diaflltered for purification. Two protein isolates were produced precipitated and soluble, with a combined protein recovery of more than 70% of total meal protein. Both products were high in protein (>85%), low in phytates (<1%), essentially free of glucosinolates (<2 (xmol/g) and had desirable functional properties comparable to those of soy protein. While the methionine content of both protein isolates was similar to the reported values, the soluble product was found to have a higher level of lysine than any canola proteins obtained before, and the precipitated protein isolate was, on the other hand, farther enriched with leucine [43]. This amino acid composition makes them suitable for nse in infant formulae (Table 4.6). Despite their excellent nutritive quality. 

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